hutH

hutH
168

histidase

Locus
BSU_39350
Molecular weight
55.51 kDa
Isoelectric point
5.22
Protein length
508 aaSequenceBlast
Gene length
1527 bpSequenceBlast
Function
histidine utilization
Product
histidase
Essential
no
E.C.
4.3.1.3
Synonyms
hutH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG2986 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon, HutP regulon, CodY regulon

Gene
Coordinates
4,042,051 → 4,043,577
The protein
Catalyzed reaction/ biological activity
L-histidine --> NH4+ + trans-urocanate (according to Swiss-Prot)
Protein family
PAL/histidase family (single member, according to UniProt)
Structure
1B8F (PDB) (from Pseudomonas putida, 43% identity, 62% similarity) PubMed
P10944 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
hutP-hutH-hutU-hutI-hutG-hutM
Description
PubMed
Regulation
induced by histidine (HutP) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
CodY: repression, PubMed, in codY regulon
HutP: antitermination, at a protein-dependent RNA switch located between hutP and hutH PubMed, in hutP regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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hutP→hutM

2025-04-03 13:30:05

ghost

154

cf3eb91928f3fe61cbc0cd204535c4f9c144314a

0AE45FCE38B0E774CFFC5F6A0A98EEDF6B02F4D4

Biological materials
Mutant
GP4202 (hutH::spec), available in  Jörg Stülke's lab
GP1114 (hutH::Tn10, spc), available in Stülke lab
BKE39350 (ΔhutH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGCCCAACTCCTTTTT,  downstream forward: _UP4_AAAGAACTAAGGGGGATGAA
BKK39350 (ΔhutH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGCCCAACTCCTTTTT,  downstream forward: _UP4_AAAGAACTAAGGGGGATGAA
References
Reviews
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Original Publications
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DB0FDF43A6829692D3E6B9CDE9D0F4AA1E3EB888

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Time of last update: 2025-04-11 21:43:20

Author of last update: MBenda