Difference between revisions of "SnaA"
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− | * '''Description:''' | + | * '''Description:''' sulphur N-acetylase, required for the conversion of S-methyl-cysteine to cysteine <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || sulphur N-acetylase |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of S-methyl-cysteine |
+ | |- | ||
+ | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29390 snaA] | ||
+ | |- | ||
+ | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/riboflavin.html Riboflavin / FAD]''' | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.508 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.508 | ||
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 534 bp, 178 aa | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 534 bp, 178 aa | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tcyJ]]'', ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tcyJ]]'', ''[[ascR]]'' |
|- | |- | ||
− | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29390 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29390 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29390 DNA_with_flanks] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ytmI_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ytmI_context.gif]] | ||
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
+ | |- | ||
+ | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ytmI_3008249_3008785_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:ytmI_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29390]] | ||
|- | |- | ||
|} | |} | ||
__TOC__ | __TOC__ | ||
+ | <br/><br/><br/><br/> | ||
+ | <br/><br/><br/><br/> | ||
+ | <br/><br/><br/><br/> | ||
+ | <br/><br/> | ||
− | + | = [[Categories]] containing this gene/protein = | |
+ | {{SubtiWiki category|[[sulfur metabolism]]}} | ||
+ | |||
+ | = This gene is a member of the following [[regulons]] = | ||
+ | {{SubtiWiki regulon|[[CymR regulon]]}}, | ||
+ | {{SubtiWiki regulon|[[AscR regulon]]}} | ||
=The gene= | =The gene= | ||
Line 35: | Line 50: | ||
=== Basic information === | === Basic information === | ||
− | * ''' | + | * '''Locus tag:''' BSU29390 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
+ | * reduced growth with S-methyl cysteine {{PubMed|23944997}} | ||
+ | * the ''[[snaA]] [[snaB]]'' double mutant does not grow with S-methyl cysteine {{PubMed|23944997}} | ||
=== Database entries === | === Database entries === | ||
Line 46: | Line 63: | ||
=== Additional information=== | === Additional information=== | ||
+ | |||
+ | |||
Line 53: | Line 72: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
+ | ** N-acetylation of the amino group of S-methyl cysteine {{PubMed|23944997}} | ||
− | * '''Protein family:''' | + | * '''Protein family:''' acetyltransferase family (according to Swiss-Prot) |
− | * '''Paralogous protein(s):''' | + | * '''Paralogous protein(s):''' [[SnaB]] |
=== Extended information on the protein === | === Extended information on the protein === | ||
Line 70: | Line 90: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
− | * '''Interactions:''' | + | * '''[[SubtInteract|Interactions]]:''' |
− | * '''Localization:''' | + | * '''[[Localization]]:''' |
=== Database entries === | === Database entries === | ||
Line 78: | Line 98: | ||
* '''Structure:''' | * '''Structure:''' | ||
− | * ''' | + | * '''UniProt:''' [http://www.uniprot.org/uniprot/O34350 O34350] |
− | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29390] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
Line 87: | Line 107: | ||
=Expression and regulation= | =Expression and regulation= | ||
+ | * '''Operon:''' ''[[snaA]]-[[tcyJ]]-[[tcyK]]-[[tcyL]]-[[tcyM]]-[[tcyN]]-[[cmoO]]-[[cmoI]]-[[cmoJ]]-[[ribR]]-[[sndA]]-[[ytnM]]'' {{PubMed|15668000}} | ||
− | * ''' | + | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ytmI_3008249_3008785_-1 snaA] {{PubMed|22383849}} |
− | * ''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|15272571}} |
* '''Regulation:''' | * '''Regulation:''' | ||
+ | ** repressed in the presence of cysteine ([[CymR]]) {{PubMed|16109943}} | ||
+ | ** induced in the presence of S-methyl-cysteine ([[AscR]]) {{PubMed|23944997}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
+ | ** [[CymR]]: transcription repression {{PubMed|16109943}} | ||
+ | ** [[AscR]]: transcription activation {{PubMed|16109943}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Line 118: | Line 143: | ||
=References= | =References= | ||
− | + | <pubmed>15937167,16109943,15668000,11390694 23944997</pubmed> | |
+ | |||
+ | [[Category:Protein-coding genes]] |
Latest revision as of 11:07, 18 August 2013
- Description: sulphur N-acetylase, required for the conversion of S-methyl-cysteine to cysteine
Gene name | ytmI |
Synonyms | |
Essential | no |
Product | sulphur N-acetylase |
Function | utilization of S-methyl-cysteine |
Gene expression levels in SubtiExpress: snaA | |
Metabolic function and regulation of this protein in SubtiPathways: Riboflavin / FAD | |
MW, pI | 20 kDa, 5.508 |
Gene length, protein length | 534 bp, 178 aa |
Immediate neighbours | tcyJ, ascR |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU29390
Phenotypes of a mutant
- reduced growth with S-methyl cysteine PubMed
- the snaA snaB double mutant does not grow with S-methyl cysteine PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- N-acetylation of the amino group of S-methyl cysteine PubMed
- Protein family: acetyltransferase family (according to Swiss-Prot)
- Paralogous protein(s): SnaB
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O34350
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Che-Man Chan, Antoine Danchin, Philippe Marlière, Agnieszka Sekowska
Paralogous metabolism: S-alkyl-cysteine degradation in Bacillus subtilis.
Environ Microbiol: 2014, 16(1);101-17
[PubMed:23944997]
[WorldCat.org]
[DOI]
(I p)
Pierre Burguière, Juliette Fert, Isabelle Guillouard, Sandrine Auger, Antoine Danchin, Isabelle Martin-Verstraete
Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism.
J Bacteriol: 2005, 187(17);6019-30
[PubMed:16109943]
[WorldCat.org]
[DOI]
(P p)
Kyle N Erwin, Shunji Nakano, Peter Zuber
Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx.
J Bacteriol: 2005, 187(12);4042-9
[PubMed:15937167]
[WorldCat.org]
[DOI]
(P p)
Irina M Solovieva, Rimma A Kreneva, Lubov Errais Lopes, Daniel A Perumov
The riboflavin kinase encoding gene ribR of Bacillus subtilis is a part of a 10 kb operon, which is negatively regulated by the yrzC gene product.
FEMS Microbiol Lett: 2005, 243(1);51-8
[PubMed:15668000]
[WorldCat.org]
[DOI]
(P p)
Jean-Yves Coppée, Sandrine Auger, Evelyne Turlin, Agnieszka Sekowska, Jean-Pierre Le Caer, Valérie Labas, Valérie Vagner, Antoine Danchin, Isabelle Martin-Verstraete
Sulfur-limitation-regulated proteins in Bacillus subtilis: a two-dimensional gel electrophoresis study.
Microbiology (Reading): 2001, 147(Pt 6);1631-1640
[PubMed:11390694]
[WorldCat.org]
[DOI]
(P p)