tcyN

tcyN
168

cystine and cysteinopantetheine ABC transporter (ATP-binding protein)

Locus
BSU_29340
Molecular weight
29.41 kDa
Isoelectric point
8.84
Protein length
259 aaSequenceBlast
Gene length
780 bpSequenceBlast
Function
cystine and cysteinopantetheine uptake
Product
cystine and cysteinopantetheine ABC transporter (ATP-binding protein)
Essential
no
Synonyms
tcyN, hisP, ytmN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1126 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,004,346 → 3,005,125
The protein
Catalyzed reaction/ biological activity
hydrolysis of ATP for the uptake of cystine and cysteinopantetheine PubMed
Protein family
ABC transporter superfamily (according to UniProt)
Domains
ABC transporter domain (aa 2-239) (according to UniProt)
Structure
2Q0H (PDB) (complex with ADP-Mg2+, Geobacillus stearothermophilus, 54%  identity )
O34900 (AlphaFold)
Paralogous protein(s)
TcyC, YxeO, PstBB, YvrO, GlnQ, ArtR, PstBA
Localization
attached to the membrane via TcyL-TcyM PubMed
Expression and Regulation
Operons
Genes
snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM
Description
PubMed
Regulation
induced in the presence of methionine and taurine PubMed
Regulatory mechanism
CymR: repression, PubMed, in cymR regulon
AscR: activation, PubMed, in ascR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

snaA→ytnM

2025-03-22 23:22:48

Jstuelk

115

8a3868e633778d2f68bf47d615f22f716e843294

4D6CA410A1DC104A491D3C18248CCD50CC0E983A

Biological materials
Mutant
GP4468 (trpC2 ΔtcyN::neo), available in Jörg Stülke's lab
GP4676 (trpC2 ΔtcyN::lox72), available in Jörg Stülke's lab
GP4659 (trpC2 ΔtcyJ-tcyK-tcyL-tcyM-tcyN::tet), available in Jörg Stülke's lab PubMed
BKE29340 (ΔtcyN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTATGGATATTTTTAATCT,  downstream forward: _UP4_GAACACATAAAGGAGCCGGT
BKK29340 (ΔtcyN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTATGGATATTTTTAATCT,  downstream forward: _UP4_GAACACATAAAGGAGCCGGT
Expression system
GP4687 (trpC2 xkdE::Pxyl-tcyJ-tcyK-tcyL-tcyM-tcyN-ermC), available in Jörg Stülke's lab
References
Loading

B24763A732111026D21C828FF9BE73FB22A123CF

Page visits: 5595

Time of last update: 2025-04-05 01:40:44

Author of last update: Jstuelk