xlyA

xlyA
168

N-acetylmuramoyl-L-alanine amidase, required for the formation of membrane vesicles in a sub-population of cells

Locus
BSU_12810
Molecular weight
31.76 kDa
Isoelectric point
5.34
Protein length
297 aaSequenceBlast
Gene length
894 bpSequenceBlast
Function
PBSX prophage-mediated lysis
Product
N-acetylmuramoyl-L-alanine amidase
Essential
no
E.C.
3.5.1.28
Synonyms
xlyA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5632 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,347,289  1,348,182
The protein
Catalyzed reaction/ biological activity
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)
Protein family
N-acetylmuramoyl-L-alanine amidase 2 family (according to UniProt)
Domains
contains a N-acetylglucosamine-polymer-binding LysM domain PubMed
contains an amidase_2 domain (like BlyA, CwlA, CwlH, XlyB)
N-acetylmuramoyl-L-alanine amidase domain (aa 45-140) (according to UniProt)
LysM domain (aa 159-203) (according to UniProt)
Structure
3HMA (PDB) PubMed
P39800 (AlphaFold)
Paralogous protein(s)
XlyB, CwlA, CwlH
Localization
extracellular (no signal peptide) PubMed
Expression and Regulation
Operons
Genes
xhlA-xhlB-xlyA
Description
PubMed
Open in new tab

xhlA→xlyA

2025-03-11 17:31:53

Jstuelk

83

8b7b23c44e6dcec6662ee55d689665ebdacc9bf7

11432F0987D78E93C6CA50E1154857E9C360C18C

Genes
xtmA-xtmB-xkdE-xkdF-xkdG-xkdH-xkdI-xkdJ-xkdK-xkdM-xkdN-xkzB-xkdO-xkdP-xkdQ-xkdR-xkdS-xkdT-xkdU-xkzA-xkdV-xkdW-xkdX-xepA-xhlA-xhlB-xlyA
Description
PubMed
Regulation
expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
Sigma factors
Xpf: sigma factor, PubMed, in xpf regulon
Open in new tab

xtmA→xlyA

2025-03-22 19:13:04

Jstuelk

142

cf834a1eded0c80a87b885b56423c31a910431c3

B52CA80EBED69B6B9017B7E04DFFD42F17910C15

Biological materials
Mutant
BKE12810 (xlyA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCATCTCTCCTTATT,  downstream forward: _UP4_TGATCAAAGACCATAAAAAT
BKK12810 (xlyA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCATCTCTCCTTATT,  downstream forward: _UP4_TGATCAAAGACCATAAAAAT
References
Reviews
Loading
Original Publications
Loading

50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0

Page visits: 3673

Time of last update: 2025-04-03 18:22:04

Author of last update: Melvin.boenninger