Difference between revisions of "PGP380"
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[[File:PGP380.jpeg|250px|thumb|right|'''pGP380: click to enlarge''']] | [[File:PGP380.jpeg|250px|thumb|right|'''pGP380: click to enlarge''']] | ||
[[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']] | [[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']] | ||
| + | '''Application:''' | ||
| − | + | The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pGP380 can be used for the [[SPINE]] method. [[pGP382]] is similar to the vector pGP380 which is derived from vectors [[pBQ200]] and [[pHT315]]. | |
| − | + | Sequencing primers: | |
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*'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘ | *'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘ | ||
*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘ | *'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘ | ||
| + | *'''FX125 (rev; primes -73bp of MCS):''' 5‘-GGCTCGTATGTTGTGTGG-3‘ | ||
| + | *'''JN54 (fwd; primes +148bp of MCS):''' 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘ | ||
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| + | <pubmed> 17994626 </pubmed> | ||
| − | + | == Return to [[Plasmids]] == | |
Latest revision as of 08:13, 29 July 2014
Application:
The vector was constructed in the Stülke lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and erythromycin in E. coli and B. subtilis, respectively. pGP380 can be used for the SPINE method. pGP382 is similar to the vector pGP380 which is derived from vectors pBQ200 and pHT315.
Sequencing primers:
- M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
- M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
- FX125 (rev; primes -73bp of MCS): 5‘-GGCTCGTATGTTGTGTGG-3‘
- JN54 (fwd; primes +148bp of MCS): 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘
Christina Herzberg, Lope Andrés Flórez Weidinger, Bastian Dörrbecker, Sebastian Hübner, Jörg Stülke, Fabian M Commichau
SPINE: a method for the rapid detection and analysis of protein-protein interactions in vivo.
Proteomics: 2007, 7(22);4032-5
[PubMed:17994626]
[WorldCat.org]
[DOI]
(P p)
