Difference between revisions of "PAC7"
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[[File:PAC7.jpeg|250px|thumb|right|'''pAC7: click to enlarge''']] | [[File:PAC7.jpeg|250px|thumb|right|'''pAC7: click to enlarge''']] | ||
| + | [[File:PAC7_cloning_region.jpeg|250px|thumb|right|'''pAC7 cloning region: click to enlarge''']] | ||
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vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site | vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site | ||
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constructed in the Rapoport lab | constructed in the Rapoport lab | ||
| − | in E. coli: ampicillin resistance | + | in ''E. coli'': ampicillin resistance |
| − | in B. subtilis: kanamycin resistance | + | in ''B. subtilis'': kanamycin resistance |
similar vectors: [[pAC5]], [[pAC6]] | similar vectors: [[pAC5]], [[pAC6]] | ||
| − | + | sequencing primer: | |
| + | *'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’ | ||
| + | *'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘ | ||
| + | *'''Kancheckrev:''' 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘ | ||
| + | |||
| + | <pubmed> 1715859 </pubmed> | ||
| + | == Return to [[Plasmids]] == | ||
Latest revision as of 13:53, 3 June 2013
vector for the construction of translational lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: kanamycin resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Kancheckrev: 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘
Y Weinrauch, T Msadek, F Kunst, D Dubnau
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.
J Bacteriol: 1991, 173(18);5685-93
[PubMed:1715859]
[WorldCat.org]
[DOI]
(P p)
