Difference between revisions of "PAC7"

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[[File:PAC7.jpeg|250px|thumb|right|'''pAC7: click to enlarge''']]
 
[[File:PAC7.jpeg|250px|thumb|right|'''pAC7: click to enlarge''']]
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[[File:PAC7_cloning_region.jpeg|250px|thumb|right|'''pAC7 cloning region: click to enlarge''']]
  
pAC7
 
  
 
vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site
 
vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site
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constructed in the Rapoport lab
 
constructed in the Rapoport lab
  
in E. coli: ampicillin resistance
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in ''E. coli'': ampicillin resistance
  
in B. subtilis: kanamycin resistance
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in ''B. subtilis'': kanamycin resistance
  
 
similar vectors: [[pAC5]], [[pAC6]]
 
similar vectors: [[pAC5]], [[pAC6]]
  
Weinrauch et al. (1991) J Bacteriol.  173:5685-5693. [http://www.ncbi.nlm.nih.gov/sites/entrez/1715859 PubMed]
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sequencing primer:
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*'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’
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*'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘
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*'''Kancheckrev:''' 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘
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 +
<pubmed> 1715859 </pubmed>
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== Return to [[Plasmids]] ==

Latest revision as of 13:53, 3 June 2013

pAC7: click to enlarge
pAC7 cloning region: click to enlarge


vector for the construction of translational lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: kanamycin resistance

similar vectors: pAC5, pAC6

sequencing primer:

  • PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
  • PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
  • Kancheckrev: 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘

Y Weinrauch, T Msadek, F Kunst, D Dubnau
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.
J Bacteriol: 1991, 173(18);5685-93
[PubMed:1715859] [WorldCat.org] [DOI] (P p)

Return to Plasmids