citA

citA
168

minor citrate synthase

Locus
BSU_09440
Molecular weight
40.78 kDa
Isoelectric point
5.78
Protein length
366 aaSequenceBlast
Gene length
1101 bpSequenceBlast
Function
unknown
Product
minor citrate synthase
Essential
no
E.C.
2.3.3.1
Synonyms
citA, citA1
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0372 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,021,057  1,022,157
The protein
Catalyzed reaction/ biological activity
acetyl-CoA + H2O + oxaloacetate --> citrate + CoA + H+ (according to UniProt)
Protein family
citrate synthase family (with MmgD and CitZ, according to UniProt)
Structure
2C6X (PDB)
P39119 (AlphaFold)
Paralogous protein(s)
MmgD, CitZ
Expression and Regulation
Operons
Genes
citA-yhdF
Description
PubMed
Regulatory mechanism
CitR: repression, PubMed, in citR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

citA→yhdF

2025-04-05 23:45:08

Jstuelk

88

1669CF6DEA93935B9FE338732703C4F1A950412D

EC50BFD2C823FA692DE52CBF1B927D137552A855

Biological materials
Mutant
GP676 (erm), available in Jörg Stülke's lab
GP1282 Δ''citA::cat'', available in Jörg Stülke's lab
GP1753 Δ(''citR citA)::aphA3, the resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis'', available in Jörg Stülke's lab
GP2360 Δ(''citR citA)::erm, the resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis'', available in Jörg Stülke's lab
BKE09440 (citA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTATTCCCCCTATCC,  downstream forward: _UP4_TAACATATTTTGGCGTTTAT
BKK09440 (citA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTATTCCCCCTATCC,  downstream forward: _UP4_TAACATATTTTGGCGTTTAT
Expression vectors
pGP2516 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
FLAG-tag construct
GP1287 (spc, based on pGP1331), available in Jörg Stülke's lab
References
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6684F6083B001E9FDC5967799B715AE966B83E1D

Page visits: 3605

Time of last update: 2025-04-08 19:28:25

Author of last update: Jstuelk