ansA

ansA
168

L-asparaginase

Locus
BSU_23580
Molecular weight
36.30 kDa
Isoelectric point
4.45
Protein length
329 aaSequenceBlast
Gene length
990 bpSequenceBlast
Function
asparagine degradation
Product
L-asparaginase
Essential
no
E.C.
3.5.1.1
Synonyms
ansA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0252 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,455,819 → 2,456,808
The protein
Catalyzed reaction/ biological activity
H2O + L-asparagine --> L-aspartate + NH4+ (according to UniProt)
Protein family
asparaginase 1 family (with AnsZ, according to UniProt)
Domains
Asparaginase/glutaminase domain (aa 2-322) (according to UniProt)
Structure
5OT0 (PDB) (from Thermococcus kodakarensis, 43% identity) PubMed
P26900 (AlphaFold)
Paralogous protein(s)
AnsZ
Expression and Regulation
Operons
Genes
ansA-ansB
Description
PubMed
Regulation
expressed in the presence of asparagine (AnsR) PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
AnsR: repression, PubMed, in ansR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

ansA→ansB

2025-04-04 08:39:03

Jstuelk

89

26B0E8E9928B9258DD0E99A3A3CCC1B9E0B8F068

0371500E4D8DAAD3D8BE963EF8C39327F0863D20

Genes
ansA-ansB-mleN-mleA
Description
PubMed
Regulation
AnsA: expressed in the presence of asparagine (AnsR) PubMed
Expression
expressed in the presence of asparagine (AnsR) PubMed
expression is reduced in motile cells as compared to non-motile cells PubMed
Regulatory mechanism
AnsR: transcription repression, PubMed, in ansR regulon
Open in new tab

ansA→mleA

2025-04-04 08:53:37

Jstuelk

102

6E6CC6ADE2F20EBE93C9F1ED4C2EF4E87AF126B1

D7F0E184034763E326DE2841E35068D2FD73DD5D

Biological materials
Mutant
GP1153 (ΔansA-ansB::ermC) available in Jörg Stülke's lab
GP4256 (ΔansA-ansB::cat) available in Jörg Stülke's lab
BKE23580 (ΔansA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACCATGCACCTCTTCAC,  downstream forward: _UP4_TAAAATGTAAAAACGATAAC
BKK23580 (ΔansA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACCATGCACCTCTTCAC,  downstream forward: _UP4_TAAAATGTAAAAACGATAAC
LacZ fusion
pGP872 (in pAC5), available in Stülke lab
References
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FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD

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Time of last update: 2025-04-06 12:30:21

Author of last update: Jstuelk