mtnE

mtnE
168

glutamine transaminase

Locus
BSU_13580
Molecular weight
43.88 kDa
Isoelectric point
4.91
Protein length
398 aaSequenceBlast
Gene length
1197 bpSequenceBlast
Function
methionine salvage
Product
glutamine transaminase
Essential
no
Synonyms
mtnE, ykrV
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,425,641  1,426,837
Phenotypes of a mutant
reduced growth with 5-methylthioribose as single sulfur source PubMed
The protein
Catalyzed reaction/ biological activity
2-ketomethylthiobutyrate + glutamine -- 2-ketoglutaramate + methionine PubMed
Protein family
class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)
Cofactors
PLP
Structure
2O1B (PDB) (from Staphylococcus aureus, 42% identity)
O31665 (AlphaFold)
Paralogous protein(s)
BacF
Expression and Regulation
Operons
Genes
mtnE
Description
PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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mtnE

2025-04-05 08:17:45

ghost

83

b576ead950c7fede3bd8b72320ba554f44c811f7

149B967F50772C291081B2EE8717DB60259EDC1D

Biological materials
Mutant
MGNA-B321 (ykrV::erm), available at the NBRP B. subtilis, Japan
BKE13580 (mtnE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATTCTCACCTTCTAA,  downstream forward: _UP4_TAACGATGGGATAATTCAAA
BKK13580 (mtnE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTATTCTCACCTTCTAA,  downstream forward: _UP4_TAACGATGGGATAATTCAAA
References
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Time of last update: 2025-04-09 05:52:22

Author of last update: Melvin.boenninger