argJ

argJ
168

N-acetylglutamate synthase

Locus
BSU_11200
Molecular weight
43.20 kDa
Isoelectric point
4.7
Protein length
406 aaSequenceBlast
Gene length
1221 bpSequenceBlast
Function
biosynthesis of arginine
Product
N-acetylglutamate synthase
Essential
no
E.C.
2.3.1.35
Synonyms
argJ, argA, argE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1364 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,196,091  1,197,311
The protein
Catalyzed reaction/ biological activity
L-glutamate + N2-acetyl-L-ornithine --> L-ornithine + N-acetyl-L-glutamate (according to UniProt)
acetyl-CoA + L-glutamate --> CoA + H+ + N-acetyl-L-glutamate (according to UniProt)
Protein family
argJ family (single member, according to UniProt)
Structure
1VZ6 (PDB) (from Streptomyces clavuligerus, 36% identity) PubMed
1VRA (PDB) (C-terminal domain, aa 193 ... 406, from B. halodurans, 60% identity)
P36843 (AlphaFold)
Modification
ArgJ has been shown to be synthesized as a precursor protein that undergoes proteolytic cleavage, leading to the formation of α and β subunits that assemble into heterodimeric or heterotetrameric molecules. PubMed
Localization
cytoplasm (according to Swiss-Prot)
Additional information
ArgJ is degraded in the forespore using MdfA as the adaptor protein PubMed
Expression and Regulation
Operons
Genes
argC-argJ-argB-argD-carA-carB-argF
Description
PubMed
Regulation
repressed by arginine (AhrC) PubMed
induced in the absence of RnpM PubMed
Regulatory mechanism
AhrC: repression, PubMed, in ahrC regulon
CodY: repression, PubMed, in codY regulon
RnpM: unknown, PubMed, in rnpM regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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argC→argF

2025-04-05 13:03:35

Jstuelk

123

bd8576e7d6b5ef98ec61ff0587a9708cd8d39155

DA268D208C03E6C5DAF0361E4714668AC025F3EA

Biological materials
Mutant
BKE11200 (argJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTTCGATTCTCTCCCG,  downstream forward: _UP4_CGCACGTAATAAAGGGGAGC
BKK11200 (argJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGTTCGATTCTCTCCCG,  downstream forward: _UP4_CGCACGTAATAAAGGGGAGC
GP3707 (argJ::aphA3 trpC2) available in the Stülke lab
Expression vectors
pGP3774: expression of Strep-argJ by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
pGP3777: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
pGP3853: expression of argJ (gapA RBS) by pBQ200 in B. subtilis, available in Jörg Stülke's lab
References
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Time of last update: 2025-04-09 04:19:59

Author of last update: Jstuelk