Difference between revisions of "FosB"

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* '''Description:''' write here <br/><br/>
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* '''Description:''' bacillithiol-S-transferase; confers resistence against antimicrobial compounds from ''B. amyloliquefaciens'' <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' ||  
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|style="background:#ABCDEF;" align="center"| '''Product''' || fosfomycin resistance protein
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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|style="background:#ABCDEF;" align="center"|'''Function''' || confers resistence against antimicrobial compounds <br/>from ''B. amyloliquefaciens''
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17840 fosB]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 6.137   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 6.137   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yndM]]'', ''[[lexA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yndM]]'', ''[[lexA]]''
 
|-
 
|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yndN_nucleotide.txt    Gene sequence     (+200bp)  ]'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17840 DNA_with_flanks]
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yndN_protein.txt Protein sequence]'''
 
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yndN_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yndN_context.gif]]
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<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fosB_1916663_1917097_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:fosB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17840]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
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 +
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<br/><br/><br/>
  
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<br/><br/>
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= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
 +
{{SubtiWiki category|[[resistance against toxins/ antibiotics]]}}
 +
 
 +
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[SigW regulon]]}}
  
 
=The gene=
 
=The gene=
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=== Basic information ===
 
=== Basic information ===
  
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* '''Coordinates:'''
+
* '''Locus tag:''' BSU17840
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17840&redirect=T BSU17840]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yndN.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yndN.html]
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
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* '''Protein family:'''
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* '''Protein family:''' FosB subfamily (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
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* '''Interactions:'''
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* '''[[SubtInteract|Interactions]]:'''  
  
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* '''Localization:'''
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* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU17840&redirect=T BSU17840]
  
 
* '''Structure:'''
 
* '''Structure:'''
  
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* '''Swiss prot entry:'''
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* '''UniProt:''' [http://www.uniprot.org/uniprot/O31817 O31817]
  
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* '''KEGG entry:'''
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17840]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:'''  
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* '''Operon:''' ''fosB'' {{PubMed|11244082}}
 +
 
 +
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fosB_1916663_1917097_1 fosB] {{PubMed|22383849}}
  
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* '''Sigma factor:'''  
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* '''Sigma factor:''' [[SigW]] {{PubMed|11244082}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** expressed under stress conditions ([[SigW]]) {{PubMed|11244082}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
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* '''Additional information:'''  
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* '''Additional information:'''
 +
** A [[ncRNA]] is predicted between ''[[fosB]]'' and ''[[lexA]]'' {{PubMed|20525796}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
 +
'''Additional publications:''' {{PubMed|20712413}}
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<pubmed>11244082,16629676, 20525796 23030527, 23256780 </pubmed>
  
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# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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[[Category:Protein-coding genes]]
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23256780

Latest revision as of 13:50, 2 April 2014

  • Description: bacillithiol-S-transferase; confers resistence against antimicrobial compounds from B. amyloliquefaciens

Gene name fosB
Synonyms yndN
Essential no
Product fosfomycin resistance protein
Function confers resistence against antimicrobial compounds
from B. amyloliquefaciens
Gene expression levels in SubtiExpress: fosB
MW, pI 17 kDa, 6.137
Gene length, protein length 432 bp, 144 aa
Immediate neighbours yndM, lexA
Sequences Protein DNA DNA_with_flanks
Genetic context
YndN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FosB expression.png

























Categories containing this gene/protein

cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics

This gene is a member of the following regulons

SigW regulon

The gene

Basic information

  • Locus tag: BSU17840

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: FosB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Alexandra A Roberts, Sunil V Sharma, Andrew W Strankman, Shayla R Duran, Mamta Rawat, Chris J Hamilton
Mechanistic studies of FosB: a divalent-metal-dependent bacillithiol-S-transferase that mediates fosfomycin resistance in Staphylococcus aureus.
Biochem J: 2013, 451(1);69-79
[PubMed:23256780] [WorldCat.org] [DOI] (I p)

Alexander P Lamers, Mary E Keithly, Kwangho Kim, Paul D Cook, Donald F Stec, Kelly M Hines, Gary A Sulikowski, Richard N Armstrong
Synthesis of bacillithiol and the catalytic selectivity of FosB-type fosfomycin resistance proteins.
Org Lett: 2012, 14(20);5207-9
[PubMed:23030527] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Bronwyn G Butcher, John D Helmann
Identification of Bacillus subtilis sigma-dependent genes that provide intrinsic resistance to antimicrobial compounds produced by Bacilli.
Mol Microbiol: 2006, 60(3);765-82
[PubMed:16629676] [WorldCat.org] [DOI] (P p)

M Cao, B A Bernat, Z Wang, R N Armstrong, J D Helmann
FosB, a cysteine-dependent fosfomycin resistance protein under the control of sigma(W), an extracytoplasmic-function sigma factor in Bacillus subtilis.
J Bacteriol: 2001, 183(7);2380-3
[PubMed:11244082] [WorldCat.org] [DOI] (P p)

23256780

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