Difference between revisions of "PHT43"
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[[File:PHT43.PNG|600px]] | [[File:PHT43.PNG|600px]] | ||
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The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''. | The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''. | ||
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* ''E. coli / B. subtilis'' shuttle vector | * ''E. coli / B. subtilis'' shuttle vector | ||
| − | pHT43 and the eight-fold protease-deficient ''B. subtilis'' strain WB800N, for secretory protein production are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)] | + | pHT43 and the eight-fold protease-deficient ''B. subtilis'' strain WB800N, for secretory protein production are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]] |
== References == | == References == | ||
<pubmed>17624574, 16005967, 16125412</pubmed> | <pubmed>17624574, 16005967, 16125412</pubmed> | ||
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The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an E. coli / B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
Features
- Secretory protein production
- Efficiently controllable gene expression (IPTG inducible)
- High level production and secretion of recombinant proteins
- E. coli / B. subtilis shuttle vector
pHT43 and the eight-fold protease-deficient B. subtilis strain WB800N, for secretory protein production are available from MoBiTec GmbH (Göttingen)
References
Hoang Duc Nguyen, Trang Thi Phuong Phan, Wolfgang Schumann
Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis.
Curr Microbiol: 2007, 55(2);89-93
[PubMed:17624574]
[WorldCat.org]
[DOI]
(P p)
Trang Thi Phuong Phan, Hoang Duc Nguyen, Wolfgang Schumann
Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis.
Protein Expr Purif: 2006, 46(2);189-95
[PubMed:16125412]
[WorldCat.org]
[DOI]
(P p)
Hoang Duc Nguyen, Quynh Anh Nguyen, Rita C Ferreira, Luis C S Ferreira, Linh Thuoc Tran, Wolfgang Schumann
Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability.
Plasmid: 2005, 54(3);241-8
[PubMed:16005967]
[WorldCat.org]
[DOI]
(P p)
