Difference between revisions of "PHT43"
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[[File:PHT43.PNG|600px]] | [[File:PHT43.PNG|600px]] | ||
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The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''. | The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σ<sup>A</sup>-dependent promoter preceding the groE operon of ''B. subtilis'' which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''. | ||
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* ''E. coli / B. subtilis'' shuttle vector | * ''E. coli / B. subtilis'' shuttle vector | ||
| − | pHT43 and the eight-fold protease-deficient ''B. subtilis'' strain WB800N, for secretory protein production are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)] | + | pHT43 and the eight-fold protease-deficient ''B. subtilis'' strain WB800N, for secretory protein production are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]] |
== References == | == References == | ||
<pubmed>17624574, 16005967, 16125412</pubmed> | <pubmed>17624574, 16005967, 16125412</pubmed> | ||
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The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an E. coli / B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
Features
- Secretory protein production
- Efficiently controllable gene expression (IPTG inducible)
- High level production and secretion of recombinant proteins
- E. coli / B. subtilis shuttle vector
pHT43 and the eight-fold protease-deficient B. subtilis strain WB800N, for secretory protein production are available from MoBiTec GmbH (Göttingen)
References
