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= Tuesday 14th April 2015 =
 
* 8:00 - 9.00 - REGISTRATION
 
* 9:00 - 9:10 – Welcome Introduction
 
  
== Session I Regulation ==
+
= Tuesday 12th June =
 +
 
 +
* 8:30 – 8:55 Registration
 +
* 8:55 Welcome - Chancellors’ Building, room 1.12
 +
 
 +
== Session 1 Regulation==
 +
CHAIR:  David Leak
 +
* 09:00 [[Ciaran Condon]]
 +
** Suppression of the essential nature of RNase M16 ([[YqfG]]), required for 3' maturation of ''B. subtilis'' 16S ribosomal RNA, by deletion of [[rnr|RNase R]]
 +
* 09:15 [[Roland Hartmann]]
 +
** Function and regulation of two 6S RNAs in Bacillus subtilis
 +
* 09:30 [[Matthias Mack]]
 +
** A dual control mechanism synchronizes riboflavin and sulphur metabolism in ''Bacillus subtilis''
 +
* 09:45 Hermann Rath
 +
** A [[SigB]]-dependent antisense RNA affects osmotic induction of ''[[opuBA|opuB]]'' expression in ''Bacillus subtilis''
 +
* 10:00 Laura Teichmann
 +
** Suppressor analysis illuminates the structure/function relationship of the glycine betaine synthesis repressor [[GbsR]]
 +
* 10:15 Sylvain Durand
 +
** sRNA-mediated activation of gene expression by inhibition of 5’-3’exonucleolytic mRNA degradation
 +
 
 +
== 10:30 – 11:00 Tea and Coffee - Chancellors’ Building, ground floor foyer==
 +
 
 +
== Session 2 [[Cell division]]==
 +
CHAIR:  TBA
 +
* 11:00 [[Henrik Strahl]]
 +
** ''Bacillus subtilis'' does not form microscopically detectable cardiolipin domains
 +
* 11:15 Jeanine Rismondo
 +
** Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanism for wall teichoic acid glycosylation
 +
* 11:30 Edward de Koning
 +
** [[Divisome]] maturation in ''Bacillus subtilis'' single cells
 +
* 11:45 Seamus Holden
 +
** Treadmilling by [[FtsZ]] filaments drives ''Bacillus subtilis'' [[cell wall synthesis|peptidoglycan synthesis]] and bacterial [[cell division]]
 +
* 12:00 [[Dirk-Jan Scheffers]]
 +
** [[PbpB|PBP2B]] [[PASTA domains]] interact with [[DivIB]] to stabilize [[cell division]]
 +
* 12:15 Yongqiang Gao
 +
** [[Cell division]] in the cell wall-less bacterium ''Acholeplasma laidlawii''
 +
 
 +
 
 +
== 12:30 – 14:30 Lunch and Poster Session 1==
 +
 
 +
== Session 3 – Interesting Biology ==
 +
CHAIR:  [[Colin Harwood]]
 +
* 14:30 [[Jörg Stülke]]
 +
** DNA topoisomerases in ''Bacillus subtilis'': A tale on mutants and suppressors
 +
* 14:45 Hannah Gaimster
 +
** Lethal depletion of essential cell envelope proteins can be rescued by slowing [[DNA replication]] in ''Bacillus subtilis''
 +
* 15:00 Alexandre Deloupy
 +
** Stochastic gene expression in Bacillus subtilis
 +
* 15:15 Sabine Schneider
 +
** Development of a reporter gene system to characterise [[riboswitch]] function and to identify [[riboswitch]] modulators by high-throughput screening
 +
* 15:30 [[Ken-ichi Yoshida]]
 +
** Rapid conjugative mobilization of a 100 kb segment of ''Bacillus subtilis'' chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer
 +
* 15:45 Anna A. Toymentseva
 +
** Mobilization and transfer of plasmids and chromosomal DNA mediated by optimized ICEBs1 conjugative element
 +
 
 +
==16:00 – 16:30 Tea and Coffee ==
 +
 +
==Session 4 – Antibiotics 1 ==
 
CHAIR:  [[Ulrike Mäder]]
 
CHAIR:  [[Ulrike Mäder]]
* 9:10 - 9:25 – [[Ken-ichi Yoshida]], Kobe, Japan
+
* 16:30 Carolin Kobras
** Hyperphosphorylation of [[DegU]] cancels [[CcpA]]-dependent catabolite repression of ''[[rocG]]'' in ''Bacillus subtilis''
+
** Exploring the substrate specificity of the antimicrobial peptide resistance transporter [[BceA]]-[[BceB]] in ''Bacillus subtilis''  
* 9:30 - 9:45 – Kambiz Morabbi Heravi, Stuttgart, DE
+
* 16:45 Luiza P. Morawska
** Activation of the ''[[glcR]]-[[ywpJ]]'' operon by phosphosugar stress in ''Bacillus subtilis''
+
** Preadaptation of ''Bacillus subtilis'' to mild osmostress contributes to increased antibiotic resistance
* 9:50 - 10:05 – Iolanda Ferro, Potsdam,DE
+
* 17:00 Mary K. Phillips-Jones
** Regulation of tRNA charging in ''Bacillus licheniformis'' and tRNA abundance
+
** Characterisation of the VanA-type VanS histidine kinase involved in glycopeptide resistance in Gram-positive bacteria and its ligand interactions
* 10:10 - 10:25 – [[Ole Økstad]], Oslo, NO
+
* 17:15 Gabriela Henriques
** Cyclic diguanylate (c-di-GMP) regulation in the ''Bacillus cereus'' group - mapping and analysis of putative c-di-GMP effectors
+
** Towards the understanding of the regulation of [[SppA]] by [[YteJ]] in Bacillus subtilis.
* 10:30 - 10:45 – Paola Randazzo, Jouy-en-Josas, FR
 
** Transcriptome and proteome analysis of the pulcherrimin-overproducing ''[[yvmB]]'' ''Bacillus subtilis'' mutant
 
  
* 10:50 – 11:15 – COFFEE BREAK
+
== Free evening in Bath ==
  
 +
= Wednesday 13th June =
  
== Session II Responses to osmotic stress ==
+
== Session 5 Biotechnology ==
CHAIR: [[Ole Økstad]]
+
CHAIR: Anne Breüner
* 11:20 - 11:35 – Sofia Arnaouteli, Heraklion, GR
+
* 9:00 Andreas Knapp
** Two putative polysaccharide deacetylases are required for osmotic stability and cell shape maintenance in ''Bacillus anthracis''
+
** The spacer region in the 5´-UTR strongly affects recombinant protein production in ''Bacillus subtilis''  
* 11:40 - 11:55 – S. Löbach, Marburg, DE
+
* 9:15 Alexandria Holland
** Osmotic control of the ''[[yqiH]]-[[yqiI]]-[[yqiK]]'' operon by the two component [[DegS]]-[[DegU]] system of ''Bacillus subtilis''
+
** Investigating protein secretion in ''Geobacillus thermoglucosidasius''  
* 12:00 - 12:15 – [[Ulrike Mäder]], Greifswald, DE
+
* 9:30 Matthew Styles
** Regulation of ''[[opuBA|opuB]]'' expression in ''Bacillus subtilis'' involves a [[SigB]]-dependent antisense RNA
+
** Engineering ''Parageobacillus'' for the Production of Terpenes: Building Better Chassis Organisms for Industrial Biotechnology
* 12:20 - 12:35 – Katja Nagler, Cologne, DE
+
* 9:45 Luca Longanesi
** Towards the identification of mechanisms of high-salinity effects on ''Bacillus subtilis'' spore [[germination]] and outgrowth
+
** γ-PGA production through Consolidated Bioprocessing in engineered ''B. subtilis'' lab strains
* 12:40 – 14:00 LUNCH (+ BACIP MEETING)
+
* 10:00 Matteo Cavaletti
 +
** Optimization of γ-PGA biosynthesis supported by synthetic biology and metabolic engineering strategies
 +
* 10:15 Ioannis Mougiakos
 +
** Characterizing a thermostable Cas9 for bacterial genome editing and silencing
  
== Session III: Focus topic: Towards a minimal cell ==
+
== 10:15 - 10:45 Tea and Coffee ==
CHAIR:  [[Jörg Stülke]]
 
* 14:00 - 14:35 – [[Josef Altenbuchner]], Stuttgart, DE
 
** Towards a ''Bacillus subtilis'' minimal genome
 
* 14:40 - 14:55 – Christopher Sauer, Newcastle, UK
 
** Influence of chromosomal location on the expression of genes in ''Bacillus subtilis''
 
* 15:00 - 15:15 –Terrens N. V. Saaki, Amsterdam, NL
 
** Constructing the Bacillus minimal [[divisome]]: what regulatory genes are really required for [[cell division]]?
 
* 15:20 - 15:35 – Etienne Dervyn, Jouy-en-Josas, FR
 
** From knowledge on gene essentiality and transcription to cell factories
 
* 15:40 - 16:00 - COFFEE BREAK
 
  
 +
== Session 6 - [[Sporulation]] and development ==
 +
CHAIR:  TBC
 +
* 10:45 [[Imrich Barák]]
 +
** What we (don’t) know about the asymmetric septum in ''Bacillus subtilis''
 +
* 11:00 Munehiro Asally
 +
** Quality monitoring during [[sporulation]] in ''B. subtilis''
 +
* 11:15 [[Ilka Bischofs]]
 +
** Phenotypic memory links different stages of the ''B. subtilis'' life cycle
 +
* 11:30 [[Akos Kovacs]]
 +
** Cheating promotes evolution of hyper-cooperators by shifting phenotypic heterogeneity in biofilms
 +
* 11:45 [[Sven Halbedel]]
 +
** Novel ''[[gpsB]]'' suppressor genes contributing to regulatory proteolysis and ''cell division''
 +
* 12:00 Sofia Arnaouteli
 +
** Exploring the role of pulcherrimin in ''Bacillus subtilis'' [[biofilm formation]]
 +
* 12:15 Karin Bjerre
 +
** Investigating of ''Bacillus subtilis'' and ''Bacillus amyloliquefaciens'' spores and vegetative cells in fecal samples from pigs
  
== Session IV: Stress & [[sporulation]] ==
+
==12:30 – 14:30 Lunch and Poster Session 2 ==
CHAIR: [[Imrich Barak]]
 
* 16:00 - 16:15 – Declan Gray, Newcastle, UK
 
** Starvation survival of ''Bacillus subtilis'' without [[sporulation]]
 
* 16:20 - 16:35 – Erwin M. Berendsen, Wageningen, NL
 
** A mobile genetic element is responsible for substantially increased heat resistance of ''Bacillus subtilis'' spores
 
* 16:40 - 16:55 – [[Kürsad Turgay]], Hannover, DE
 
** Thermotolerance development in ''Bacillus subtilis''
 
* 17:00 - 17:15– Marina Raguse, Cologne, DE
 
** Plasma sterilization as an innovative tool to inactive ''Bacillus subtilis'' endospores
 
* 17:20 - 17:35 – Alexander Reder, Greifswald, DE
 
** Issues of competence – the general stress [[sigma factor]] [[SigB]] silences competence development in ''Bacillus subtilis''
 
* 17.40 - 17.55—[[Jan Maarten van Dijl]], Groningen, NL
 
** [[Ribosome]] remodeling in ethanol-stressed cells of ''Bacillus subtilis'' is mediated by a [[SigB]]-dependent antisense RNA
 
* 18.00 - 18.15—[[Sylvie Nessler]], University Paris-Sud, FR
 
** ''Bacillus subtilis'' serine/threonine protein kinase [[YabT]]
 
  
== 18.20 - 19.00 BACELL STEERING GROUP MEETING ==
+
==Session 7 – Antibiotics 2 ==
 +
CHAIR:  [[Kevin Devine]]
 +
* 14:30 Auke J. van Heel
 +
** BAGEL4: A user-friendly web server to mine RiPPs and bacteriocins
 +
* 14:45 Amanda Y. van Tilburg
 +
** ''Bacillus subtilis'' as heterologous production host for lantibiotics
 +
* 15:00 Nina Lautenschlaeger
 +
** A novel heterologous whole-cell biosensor in ''Bacillus subtilis'' for the comprehensive detection of b-lactam antibiotics
 +
* 15:15 Marjorie Gibbon
 +
** Towards understanding signalling between the bacitracin resistance transporter [[BceA]]-[[BceB]] and histidine kinase [[BceS]] of ''Bacillus subtilis''
  
= Wednesday April 15th 2015 =
+
== 15:30 16:00 Tea and Coffee ==
== Session V: Tools et al. ==
 
CHAIR: [[Stephane Aymerich]]
 
* 9:00 - 9:15 – Jara Radeck, Munich, DE
 
** The ''Bacillus'' BioBrick Box 2.0: Generation and Evaluation of New Essential Genetic Building Blocks for Standardized Work with ''Bacillus subtilis''
 
* 9:20 - 9:35 Wilfried J. J. Meijer, Madrid, ES
 
** Studying ''Bacillus subtilis'' plasmid conjugation and its use as a tool for modifying other relevant Gram positive bacteria
 
* 9:40 - 9:55 – Trupti Sharma, Bath, UK
 
** Microbiologically induced calcium carbonate precipitation by extremophilic ''Bacillus'' species – A potential application as microbial agents for the development of self-healing concrete
 
* 10:00 - 10:35 - COFFEE BREAK
 
  
== Session VI: Cell wall and cell biology ==
+
== Session 8 – Cereus Group ==
CHAIR: [[Uwe Völker]]
+
CHAIR: [[Cinzia Calvio]]
* 10:40 - 10:55 – Mike van Nieuwenhze, Bloomington/ Newcastle, USA/ UK
+
* 16:00 Monika Ehling-Schulz
** Novel chemical tools for probing the dynamics of peptidoglycan biosynthesis
+
** ''Bacillus cereus'', a multifaceted pathogen: The challenge of discriminating high and low enteropathogenic strains
* 11:00 - 11:15 – Henrik Strahl, Newcastle, UK
+
* 16:15 Nick Waterfield
** Polar localization of bacterial chemoreceptors by recruitment to highly curved septal membrane
+
** ''Bacillus cereus'' G9241 – to anthrax and back again
* 11:20 - 11:35 – David Noone, Trinity College Dublin, IE
+
* 16:30 Ludivine Rousset
** The [[WalK]] sensor kinase detects several signals during the growth cycle of ''Bacillus subtilis''
+
** Oligotrophy induces phenotypic diversification of ''Bacillus cereus'' AH187
* 11:40 - 11:55 – Karzan R. Sidiq, Newcastle, UK
+
* 16:45 Jan Maarten van Dijl
** A D-alanine transporter in ''Bacillus subtilis''
+
** Can ''Bacillus thuringiensis'' and other microorganisms isolated from chronic wounds ‘pacify’ ''Staphylococcus aureus''?
* 12:00 - 12:15 – [[Peter Graumann]], Marburg, DE
 
** [[MreB]] forms curved sheets of filaments that nucleate on membranes ''in vitro''
 
* 12:20 - 12:35 – Jeanine Rismondo, Wernigerode, DE
 
** Discrete and overlapping roles of the five high molecular weight penicillin binding proteins in [[cell division]], growth and virulence of ''Listeria monocytogenes''
 
** 12:40 - 13:40 – LUNCH
 
  
== Session VII: Toxins & antibiotics/ Protection & Resistance ==
+
== 17:00 Closing Remarks==
CHAIR: Sandrine Auger
 
* 13:40 - 13:55 – N. Jahn, Jena, DE
 
** Against the mainstream: The type I toxin [[BsrG]] from ''Bacillus subtilis'' does not disrupt membrane potential although it is recruited to the cell membrane
 
* 14:00 - 14:15 –Yazen Yaseen, Lille, FR
 
** Comparative study of fengycin promoters using green fluorescent protein (GFP) fusion marker
 
* 14:20 - 14:35 –Luminita Ciolacu, KEMIN, BE
 
** Lipopeptides of ''Bacillus subtilis'' PB6 - A comparison of two phenotypes
 
* 14:40 - 14:55—Elrike Frenzel, Groningen, NL
 
** Single cell profiling of cereulide toxin producing ''Bacillus cereus''
 
* 15:00 - 15:15 – [[Anne-Brit Kolsto|Anne-Brit Kolstø]], Oslo, NO
 
** The secret lives of bacterial efflux transporter proteins
 
* 15:20 - 15:35 – [[Susanne Gebhard]], Bath, UK
 
** Need-based activation of antibiotic resistance by a flux-sensing mechanism
 
* 15:40 - 16:00 – COFFEE BREAK
 
  
== Session VIII: Biofilm & Secretion ==
+
== 19:00 – 23:00 Social event at The Terrace, The Roman Baths, generously supported by BACIP – to include the prize giving for best posters and talks from PhD/Postdocs ==
CHAIR: [[Kevin Devine]]
 
* 16:00 - 16:15 – [[Akos T Kovacs]], University of Jena, DE
 
** Living on the surface: [[Biofilm formation]] and surface motility of ''Bacillus subtilis''
 
* 16:20 - 16:35 – Cinzia Calvio, University of Pavia, IT
 
** Four “y” genes in ''B. subtilis'' genome encode γ-PGA hydrolases
 
* 16:40 - 16:55 Stavroula Balomenou, University of Crete, GR
 
** Distinct functions of polysaccharide deacetylases in physiology, virulence and [[biofilm formation]] of ''Bacillus anthracis''
 
* 17:00 - 17:15 – Rita Cruz, DSM Delft, NL
 
** Impact of secretion and wall stress on [[protein secretion]] by ''Bacillus subtilis''
 
* 17:20 - 17:35 – Alexandria Holland, Bath University, UK
 
** [[Protein secretion]] in ''Bacillus thermoglucosidasius''
 
* 17:40 - 17:55 Sara Kesel, University of Munich, DE
 
** Carbohydrate-coating reduces adhesion of biofilm forming ''Bacillus subtilis'' to gold surfaces
 
 
* 18:00 Drinks
 
  
* 19:00 Dinner
 
  
 
= END OF MEETING =
 
= END OF MEETING =

Latest revision as of 15:38, 4 June 2018

Tuesday 12th June

  • 8:30 – 8:55 Registration
  • 8:55 Welcome - Chancellors’ Building, room 1.12

Session 1 Regulation

CHAIR: David Leak

  • 09:00 Ciaran Condon
    • Suppression of the essential nature of RNase M16 (YqfG), required for 3' maturation of B. subtilis 16S ribosomal RNA, by deletion of RNase R
  • 09:15 Roland Hartmann
    • Function and regulation of two 6S RNAs in Bacillus subtilis
  • 09:30 Matthias Mack
    • A dual control mechanism synchronizes riboflavin and sulphur metabolism in Bacillus subtilis
  • 09:45 Hermann Rath
    • A SigB-dependent antisense RNA affects osmotic induction of opuB expression in Bacillus subtilis
  • 10:00 Laura Teichmann
    • Suppressor analysis illuminates the structure/function relationship of the glycine betaine synthesis repressor GbsR
  • 10:15 Sylvain Durand
    • sRNA-mediated activation of gene expression by inhibition of 5’-3’exonucleolytic mRNA degradation

10:30 – 11:00 Tea and Coffee - Chancellors’ Building, ground floor foyer

Session 2 Cell division

CHAIR: TBA

  • 11:00 Henrik Strahl
    • Bacillus subtilis does not form microscopically detectable cardiolipin domains
  • 11:15 Jeanine Rismondo
    • Discovery of genes required for lipoteichoic acid glycosylation predicts two distinct mechanism for wall teichoic acid glycosylation
  • 11:30 Edward de Koning
    • Divisome maturation in Bacillus subtilis single cells
  • 11:45 Seamus Holden
  • 12:00 Dirk-Jan Scheffers
  • 12:15 Yongqiang Gao
    • Cell division in the cell wall-less bacterium Acholeplasma laidlawii


12:30 – 14:30 Lunch and Poster Session 1

Session 3 – Interesting Biology

CHAIR: Colin Harwood

  • 14:30 Jörg Stülke
    • DNA topoisomerases in Bacillus subtilis: A tale on mutants and suppressors
  • 14:45 Hannah Gaimster
    • Lethal depletion of essential cell envelope proteins can be rescued by slowing DNA replication in Bacillus subtilis
  • 15:00 Alexandre Deloupy
    • Stochastic gene expression in Bacillus subtilis
  • 15:15 Sabine Schneider
    • Development of a reporter gene system to characterise riboswitch function and to identify riboswitch modulators by high-throughput screening
  • 15:30 Ken-ichi Yoshida
    • Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer
  • 15:45 Anna A. Toymentseva
    • Mobilization and transfer of plasmids and chromosomal DNA mediated by optimized ICEBs1 conjugative element

16:00 – 16:30 Tea and Coffee

Session 4 – Antibiotics 1

CHAIR: Ulrike Mäder

  • 16:30 Carolin Kobras
    • Exploring the substrate specificity of the antimicrobial peptide resistance transporter BceA-BceB in Bacillus subtilis
  • 16:45 Luiza P. Morawska
    • Preadaptation of Bacillus subtilis to mild osmostress contributes to increased antibiotic resistance
  • 17:00 Mary K. Phillips-Jones
    • Characterisation of the VanA-type VanS histidine kinase involved in glycopeptide resistance in Gram-positive bacteria and its ligand interactions
  • 17:15 Gabriela Henriques
    • Towards the understanding of the regulation of SppA by YteJ in Bacillus subtilis.

Free evening in Bath

Wednesday 13th June

Session 5 Biotechnology

CHAIR: Anne Breüner

  • 9:00 Andreas Knapp
    • The spacer region in the 5´-UTR strongly affects recombinant protein production in Bacillus subtilis
  • 9:15 Alexandria Holland
    • Investigating protein secretion in Geobacillus thermoglucosidasius
  • 9:30 Matthew Styles
    • Engineering Parageobacillus for the Production of Terpenes: Building Better Chassis Organisms for Industrial Biotechnology
  • 9:45 Luca Longanesi
    • γ-PGA production through Consolidated Bioprocessing in engineered B. subtilis lab strains
  • 10:00 Matteo Cavaletti
    • Optimization of γ-PGA biosynthesis supported by synthetic biology and metabolic engineering strategies
  • 10:15 Ioannis Mougiakos
    • Characterizing a thermostable Cas9 for bacterial genome editing and silencing

10:15 - 10:45 Tea and Coffee

Session 6 - Sporulation and development

CHAIR: TBC

  • 10:45 Imrich Barák
    • What we (don’t) know about the asymmetric septum in Bacillus subtilis
  • 11:00 Munehiro Asally
  • 11:15 Ilka Bischofs
    • Phenotypic memory links different stages of the B. subtilis life cycle
  • 11:30 Akos Kovacs
    • Cheating promotes evolution of hyper-cooperators by shifting phenotypic heterogeneity in biofilms
  • 11:45 Sven Halbedel
    • Novel gpsB suppressor genes contributing to regulatory proteolysis and cell division
  • 12:00 Sofia Arnaouteli
  • 12:15 Karin Bjerre
    • Investigating of Bacillus subtilis and Bacillus amyloliquefaciens spores and vegetative cells in fecal samples from pigs

12:30 – 14:30 Lunch and Poster Session 2

Session 7 – Antibiotics 2

CHAIR: Kevin Devine

  • 14:30 Auke J. van Heel
    • BAGEL4: A user-friendly web server to mine RiPPs and bacteriocins
  • 14:45 Amanda Y. van Tilburg
    • Bacillus subtilis as heterologous production host for lantibiotics
  • 15:00 Nina Lautenschlaeger
    • A novel heterologous whole-cell biosensor in Bacillus subtilis for the comprehensive detection of b-lactam antibiotics
  • 15:15 Marjorie Gibbon
    • Towards understanding signalling between the bacitracin resistance transporter BceA-BceB and histidine kinase BceS of Bacillus subtilis

15:30 – 16:00 Tea and Coffee

Session 8 – Cereus Group

CHAIR: Cinzia Calvio

  • 16:00 Monika Ehling-Schulz
    • Bacillus cereus, a multifaceted pathogen: The challenge of discriminating high and low enteropathogenic strains
  • 16:15 Nick Waterfield
    • Bacillus cereus G9241 – to anthrax and back again
  • 16:30 Ludivine Rousset
    • Oligotrophy induces phenotypic diversification of Bacillus cereus AH187
  • 16:45 Jan Maarten van Dijl
    • Can Bacillus thuringiensis and other microorganisms isolated from chronic wounds ‘pacify’ Staphylococcus aureus?

17:00 Closing Remarks

19:00 – 23:00 Social event at The Terrace, The Roman Baths, generously supported by BACIP – to include the prize giving for best posters and talks from PhD/Postdocs

END OF MEETING

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