Difference between revisions of "RoxS"

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(Created page with "* '''Description:''' small RNA<br/><br/> {| align="right" border="1" cellpadding="2" |- |style="background:#ABCDEF;" align="center"|'''Gene name''' |''roxS'' |- |style="back...")
 
 
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* '''Description:''' small RNA<br/><br/>
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* '''Description:''' small RNA, controls expression of ''[[ppnKB]]'' and ''[[sucC]]-[[sucD]]''<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' ||  
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|style="background:#ABCDEF;" align="center"| '''Product''' || trans-acting regulatory RNA
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' ||  
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of ''[[ppnKB]]'' and ''[[sucC]]-[[sucD]]'' expression
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Gene length''' ||   
 
|style="background:#ABCDEF;" align="center"| '''Gene length''' ||   
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[ResD regulon]]}}
  
 
+
= The [[RoxS regulon]] =
 +
* ''[[ppnKB]]'', ''[[sucC]]-[[sucD]]''
 
=The gene=
 
=The gene=
  
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* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
 +
 +
=The RNA=
 +
 +
=== Basic information/ Evolution ===
 +
 +
* '''Catalyzed reaction/ biological activity:''' hybridizes to ''[[ppnKB]]'' and ''[[sucD]]'' mRNAs to inhibit their translation and to destabilize them {{PubMed|25643072}}
 +
 +
=== Extended information on the RNA===
 +
 +
* '''Kinetic information'''
 +
 +
* '''[[SubtInteract|Interactions]]:'''
 +
** binds ''[[ppnKB]]'', and ''[[sucC]]-[[sucD]]'' mRNAs {{PubMed|25643072}}
 +
 +
* '''[[Localization]]'''
 +
 +
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=?&redirect=T ?]
 +
 +
* '''Structure''':
 +
 +
=== Additional information===
  
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''roxS''   
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* '''Operon:''' ''roxS''  {{PubMed|22383849}}
  
 
* '''[[Sigma factor]]:'''  
 
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** induced by nitric oxide (NO) ([[ResD]]) {{PubMed|25643072}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[ResD]]: transcription activation {{PubMed|25643072}}
  
* '''Additional information:'''  
+
* '''Additional information:'''
 +
** the roxS RNA is degraded by the joined activities of [[rny|RNase Y]] (initial endonucleolytic cleavage) and [[rnjA|RNase J1]] (processive degradation) {{PubMed|25643072}}
 +
** the ''[[roxS]]''-''[[ppnKB]]'' and ''[[roxS]]-[[sucC]]'' RNA duplexes form a cleavage site for [[rnc|RNase III]] {{PubMed|25643072}}
  
 
=Biological materials =
 
=Biological materials =
  
 
* '''Mutant:'''
 
* '''Mutant:'''
 
+
** available in [[Ciaran Condon]]'s lab {{PubMed|25643072}}
 
* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          

Latest revision as of 12:37, 4 February 2015

  • Description: small RNA, controls expression of ppnKB and sucC-sucD

Gene name roxS
Synonyms rsaE
Essential no
Product trans-acting regulatory RNA
Function control of ppnKB and sucC-sucD expression
Gene length
Immediate neighbours yizD, yjbH
Gene sequence (+200bp)
Genetic context








Categories containing this gene/protein

ncRNA

This gene is a member of the following regulons

ResD regulon

The RoxS regulon

The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • BsubCyc: no entry
  • DBTBS entry: no entry
  • SubtiList entry: no entry
  • Structure:

Additional information

  • Similar RNA:

The RNA

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: hybridizes to ppnKB and sucD mRNAs to inhibit their translation and to destabilize them PubMed

Extended information on the RNA

  • Kinetic information

Database entries

  • BsubCyc: ?
  • Structure:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:

Biological materials

  • lacZ fusion:


Labs working on this gene/RNA

Ciaran Condon, Paris

Your additional remarks

References

Sylvain Durand, Frédérique Braun, Efthimia Lioliou, Cédric Romilly, Anne-Catherine Helfer, Laurianne Kuhn, Noé Quittot, Pierre Nicolas, Pascale Romby, Ciarán Condon
A nitric oxide regulated small RNA controls expression of genes involved in redox homeostasis in Bacillus subtilis.
PLoS Genet: 2015, 11(2);e1004957
[PubMed:25643072] [WorldCat.org] [DOI] (I e)