clpE

clpE
168

AAA unfoldase, ATPase subunit of the ClpE-ClpP protease (class III stress gene)

Locus
BSU_13700
Molecular weight
77.72 kDa
Isoelectric point
5.14
Protein length
699 aaSequenceBlast
Gene length
2100 bpSequenceBlast
Function
protein degradation
Product
AAA unfoldase, ATPase subunit of the ClpE-ClpP protease
Essential
no
Synonyms
clpE, ykvH
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0542 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,435,628  1,437,727
The protein
Catalyzed reaction/ biological activity
ATPase/chaperone
Protein family
ClpA/ClpB family (with ClpC, according to UniProt)
Domains
AAA-ATPase PFAM
UVR domain (aa 325-360) (according to UniProt)
Structure
6EM8 (PDB) (ClpC from Staphylococcus aureus, 54% identity) PubMed
O31673 (AlphaFold)
Paralogous protein(s)
ClpC
Localization
localization in cytoplasmic polar clusters, excluded from the nucleoid, colocalization with ClpP Pubmed
forms foci coincident with nucleoid edges, usually near cell poles PubMed
Expression and Regulation
Operons
Genes
clpE
Description
PubMed
Regulation
induced by heat (CtsR) PubMed
Regulatory mechanism
CtsR: repression, PubMed, in ctsR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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clpE

2025-04-08 14:45:10

ghost

81

70ee23115086270596dd5085fcef39e59d159f88

CD123E8BF4C8D5620B75122505863C86AD11D257

Biological materials
Mutant
GP3784 (ΔclpE::neo) available in Jörg Stülke's lab
clpE::spec available from the Hamoen] Lab
BKE13700 (clpE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCCAAAACCTCCTTA,  downstream forward: _UP4_TAACAATCAGCGGTTTCCTT
BKK13700 (clpE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCCAAAACCTCCTTA,  downstream forward: _UP4_TAACAATCAGCGGTTTCCTT
Expression vectors
pGP3695 (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3925: expression of clpE by pBQ200 in B. subtilis, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP1799 (spc, based on pGP1331), available in Jörg Stülke's lab
Antibody
available in Ulf Gerth's and Jörg Stülke's labs
GFP fusion
C-terminal YFP and CFP fusions (single copy) available from the Hamoen] Lab
References
Reviews
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Original Publications
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Labs working on this gene/protein
Leendert Hamoen, Newcastle University, UK homepage

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Page visits: 5954

Time of last update: 2025-04-11 16:16:59

Author of last update: Dennis.wicke