ykuN

ykuN
168

flavodoxin, binds FMN, replaces ferredoxin under conditions of iron limitation, probably involved in electron transfer to nitric oxide synthase

Locus
BSU_14150
Molecular weight
17.65 kDa
Isoelectric point
3.83
Protein length
158 aaSequenceBlast
Gene length
477 bpSequenceBlast
Function
electron transfer
Product
flavodoxin
Essential
no
Synonyms
ykuN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0716 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,487,038  1,487,514
The protein
Protein family
flavodoxin family (with YkuP, according to UniProt)
Domains
Flavodoxin-like domain (aa 4-144) (according to UniProt)
Cofactors
FMN PubMed
Structure
5LJL (PDB) (flavodoxin from Streptococcus pneumoniae, 42% identity) PubMed
O34737 (AlphaFold)
Paralogous protein(s)
YkuP
Expression and Regulation
Operons
Genes
ykuN-ykuO-ykuP
Description
PubMed
Regulation
expressed under anaerobic conditions (ResD) PubMed
induced by iron starvation (second wave) (Fur) PubMed
Regulatory mechanism
ResD: activation, PubMed, in resD regulon
Kre: repression, in kre regulon
NsrR: repression, PubMed, in nsrR regulon
Fur: repression, PubMed, in fur regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigI: sigma factor, PubMed, in sigI regulon
Additional information
the ''YkuN-YkuO-YkuP operon is strongly unregulated in a kre'' mutant PubMed
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ykuN→ykuP

2025-04-08 16:20:55

Jstuelk

98

57db5b9bbc0032750db2eb9bfdbed1110c747af0

E599E3DBF7B4049028BE0EE09C6A470E8B1A923E

Biological materials
Mutant
GP4733 ΔykuN::aphA3, available in Jörg Stülke's lab
MGNA-B337 (ykuN::erm), available at the NBRP B. subtilis, Japan
BKE14150 (ykuN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTATCACCCCATTAGT,  downstream forward: _UP4_GATTATATGAACAAGGAAAA
BKK14150 (ykuN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTATCACCCCATTAGT,  downstream forward: _UP4_GATTATATGAACAAGGAAAA
References
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0932DDC285635C02067A3302CEDA302FE4269179

Page visits: 6293

Time of last update: 2025-04-12 09:29:48

Author of last update: Jstuelk