wapA

wapA
168

cell wall-associated protein precursor, contact-dependent growth inhibition protein

Locus
BSU_39230
Molecular weight
258.02 kDa
Isoelectric point
6.13
Protein length
2334 aaSequenceBlast
Gene length
7005 bpSequenceBlast
Function
intercellular competition
Product
cell wall-associated protein precursor
Essential
no
Synonyms
wapA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3209 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,023,544 → 4,030,548
Additional information
the gene readily acquires mutations during cultivation with Arabidopsis thaliana roots PubMed
The protein
Catalyzed reaction/ biological activity
the C-terminal toxic domain has RNase activity (cleaves tRNAs) PubMed
Protein family
RHS/WapA nuclease family (single member, according to UniProt)
Domains
cell wall binding domain as retention signal
C-terminal toxic domain with RNase activity (cleaves tRNAs) PubMed
Has two ligand-binding domains; the N-terminus has three 101 AA repeats which are responsible for cell wall binding; the C-terminus consists of two blocks of residues with a conserved motif repeated 31 times (according to UniProt)
Structure
Q07833 (AlphaFold)
Effectors of protein activity
WapI inhibits the toxic activity of WapA PubMed
Localization
extracellular (signal peptide), cell wall binding domain as retention signal, major constituent of the secretome PubMed
Expression and Regulation
Operons
Genes
wapA-wapI
Description
PubMed
Regulation
repressed at high salt concentration (DegU-P) PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
DegU: repression, DegU-P, PubMed, in degU regulon
YvrHb: activation, PubMed, in yvrHb regulon
WalR: repression, PubMed, in walR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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wapA→wapI

2025-04-07 04:22:59

Jstuelk

159

ACCB16F95EA75BBC03E1DD4186EAB71F0DEDFADE

84D822AFD36349840C746076174D4353AE6A1D3C

Biological materials
Mutant
BKE39230 (ΔwapA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCTCTCTCCTTTTGT,  downstream forward: _UP4_TAATAAGGTTAAGCGAGGGG
BKK39230 (ΔwapA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCTCTCTCCTTTTGT,  downstream forward: _UP4_TAATAAGGTTAAGCGAGGGG
References
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BBC06DA57CAC2B2378763E0839C6C31AFBEA0417

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Time of last update: 2025-04-09 00:05:10

Author of last update: Jstuelk