Difference between revisions of "PAC7"
(New page: vector for constructed in the --- lab in E. coli: ampicillin resistance in B. subtilis: ---- resistance based on --- reference. PubMed) |
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| − | vector for | + | [[File:PAC7.jpeg|250px|thumb|right|'''pAC7: click to enlarge''']] |
| − | constructed in the | + | [[File:PAC7_cloning_region.jpeg|250px|thumb|right|'''pAC7 cloning region: click to enlarge''']] |
| − | in E. coli: ampicillin resistance | + | |
| − | in B. subtilis: ---- | + | |
| − | + | vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site | |
| − | + | ||
| + | does not replicate in ''B. subtilis'', integrates into the ''amyE'' site | ||
| + | |||
| + | constructed in the Rapoport lab | ||
| + | |||
| + | in ''E. coli'': ampicillin resistance | ||
| + | |||
| + | in ''B. subtilis'': kanamycin resistance | ||
| + | |||
| + | similar vectors: [[pAC5]], [[pAC6]] | ||
| + | |||
| + | sequencing primer: | ||
| + | *'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’ | ||
| + | *'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘ | ||
| + | *'''Kancheckrev:''' 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘ | ||
| + | |||
| + | <pubmed> 1715859 </pubmed> | ||
| + | == Return to [[Plasmids]] == | ||
Latest revision as of 13:53, 3 June 2013
vector for the construction of translational lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: kanamycin resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Kancheckrev: 5‘-CTGCCTCCTCATCCTCTTCATCC-3‘
Y Weinrauch, T Msadek, F Kunst, D Dubnau
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis.
J Bacteriol: 1991, 173(18);5685-93
[PubMed:1715859]
[WorldCat.org]
[DOI]
(P p)
