Difference between revisions of "PAC6"
(New page: vector for constructed in the --- lab in E. coli: ampicillin resistance in B. subtilis: ---- resistance based on --- reference. PubMed) |
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| − | vector for | + | [[File:PAC6.jpeg|250px|thumb|right|'''pAC6: click to enlarge''']] |
| − | constructed in the | + | |
| − | in E. coli: ampicillin resistance | + | vector for the construction of transcriptional ''lacZ'' fusions that can be integrated at the ''amyE'' site |
| − | in B. subtilis: ---- | + | |
| − | + | does not replicate in ''B. subtilis'', integrates into the ''amyE'' site | |
| − | + | ||
| + | constructed in the Rapoport lab | ||
| + | |||
| + | in ''E. coli'': ampicillin resistance | ||
| + | |||
| + | in ''B. subtilis'': chloramphenicol resistance | ||
| + | |||
| + | similar vectors: [[pAC5]], [[pAC7]] | ||
| + | |||
| + | sequencing primer: | ||
| + | *'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’ | ||
| + | *'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘ | ||
| + | *'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’ | ||
| + | |||
| + | <pubmed> 11902727 </pubmed> | ||
| + | == Return to [[Plasmids]] == | ||
Latest revision as of 13:53, 3 June 2013
vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site
does not replicate in B. subtilis, integrates into the amyE site
constructed in the Rapoport lab
in E. coli: ampicillin resistance
in B. subtilis: chloramphenicol resistance
sequencing primer:
- PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
- PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
- Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
J Stülke, I Martin-Verstraete, M Zagorec, M Rose, A Klier, G Rapoport
Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT.
Mol Microbiol: 1997, 25(1);65-78
[PubMed:11902727]
[WorldCat.org]
[DOI]
(P p)
