rnjA
168
RNase J1, important for the degradation of RNA fragments containing transcription terminators, resolves stalled transcription complexes
Locus
BSU_14530
Molecular weight
61.34 kDa
Isoelectric point
5.9
Function
RNA processing
Product
RNase J1
Essential
no
Synonyms
rnjA, ykqC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0595 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,523,118 1,524,785
Phenotypes of a mutant
growth is strongly impaired (doubling time in complex medium 145 minutes vs. 43 minutes for the wild type) PubMed
the mutant is strongly impaired in sporulation PubMed
loss of genetic competence PubMed
The protein
Catalyzed reaction/ biological activity
endonuclease and 5'-3' exonuclease
resolves stalled transcription complexes by a “torpedo” mechanism, whereby RNase J1 degrades the nascent RNA and causes the transcription complex to disassemble upon collision with RNA polymerase PubMed
Protein family
metallo-beta-lactamase superfamily (according to UniProt)
Structure
Effectors of protein activity
a NAD-cap stabilizes RNA against exonucleolytic attack by RNase J1 PubMed
Paralogous protein(s)
cytoplasm, colocalizes with the ribosomes PubMed
Additional information
Expression and Regulation
Operons
Biological materials
Mutant
GP41 (rnjA under control of p(xyl)), available in Jörg Stülke's lab
SSB342 (rnjA under pspac), cat, available in Harald Putzer's lab
Expression vectors
for chromosomal expression of RNase J1-Strep (spc): GP1034, available in Jörg Stülke's lab
for chromosomal expression of RNase J1-Strep (cat): GP1042, available in Jörg Stülke's lab
pGP1441: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed
FLAG-tag construct
GP1020 (spc, based on pGP1331), available in Jörg Stülke's lab
GP1075 (aphA3), available in Jörg Stülke's lab
LacZ fusion
pGP418 (in pAC7), available in Jörg Stülke's lab
GFP fusion
GP1694 (in pHJS-105 PubMed), expression of rnjA-sfGFP::spc under a xylose-inducible promoter in B. subtilis, available in Jörg Stülke's lab PubMed
GP1722 (in pBP43), expression of rnjA-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed
Labs working on this gene/protein
Harald Putzer, IBPC Paris, France Homepage
David Bechhofer, Mount Sinai School, New York, USA Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage
Ciaran Condon, IBPC, Paris, France Homepage
References
Reviews
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Original Publications
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Page visits: 9735
Time of last update: 2025-04-08 14:56:25
Author of last update: Jstuelk