Difference between revisions of "PGP1459"

From SubtiWiki
Jump to: navigation, search
 
(2 intermediate revisions by 2 users not shown)
Line 8: Line 8:
  
 
Sequencing primers:  
 
Sequencing primers:  
*'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘
+
*'''KG64:''' 5‘-TATCAGGGCCTCGACTACA-3‘
*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘
+
*'''ML107:''' 5‘-GCTTCATAGAGTAATTCTGTAAAGG-3‘
  
 
Similar plasmid: [[pGP1460]] (for C-terminal Strep-tags)
 
Similar plasmid: [[pGP1460]] (for C-terminal Strep-tags)
  
<pubmed>  </pubmed>
+
<pubmed> 23192352 </pubmed>
 +
 
 +
==  Return to [[Plasmids]] ==

Latest revision as of 13:58, 3 June 2013

PGP1459: click to enlarge
pGP380 cloning region: click to enlarge

The vector was constructed in the Stülke lab and it is suitable for constitutive expression of N-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI. The plasmid will integrate into the ganA gene. Just like pGP380 it can be used for the SPINE method.

The cloning region of pGP380 is shown for detailed information.


Sequencing primers:

  • KG64: 5‘-TATCAGGGCCTCGACTACA-3‘
  • ML107: 5‘-GCTTCATAGAGTAATTCTGTAAAGG-3‘

Similar plasmid: pGP1460 (for C-terminal Strep-tags)

Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352] [WorldCat.org] [DOI] (I p)


Return to Plasmids