purH
168

phosphoribosylaminoimidazole carboxamide formyltransferase

Locus

BSU_06520

Molecular weight

55.57 kDa

Isoelectric point

5.13

Protein length

512 aaSequenceBlast

Gene length

1539 bpSequenceBlast

Function

purine biosynthesis

Product

phosphoribosylaminoimidazole carboxamide formyltransferaseand inosine-monophosphate cyclohydrolase

Essential

E.C.

2.1.2.3

Synonyms

purH, purJ

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0138 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

708,594 710,132

Phenotypes of a mutant

more resistant than wild-type to zinc limitation when grown on rich medium (LB), this is blocked by simultaneous deletion of purB (reason: accumulation of ZTP that stimulates the binding of ZagA to FolE, and thus folate biosynthesis during zinc limitation) PubMed

The protein

Catalyzed reaction/ biological activity

(6S)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamide --> (6S)-5,6,7,8-tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)

H₂O + IMP --> 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (according to UniProt)

Protein family

purH family (single member, according to UniProt)

Domains

MGS-like domain (aa 1-146) (according to UniProt)

Structure

1ZCZ (PDB) (from Thermotoga maritima, 36% identity, 54% similarity) PubMed

P12048 (AlphaFold)

Additional information

belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons