mdh

mdh
168

malate dehydrogenase

Locus
BSU_29120
Molecular weight
33.49 kDa
Isoelectric point
4.73
Protein length
Gene length
Function
TCA cycle
Product
malate dehydrogenase
Essential
no
E.C.
1.1.1.37
Synonyms
mdh, citH

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0039 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,978,734 2,979,672
The protein
Catalyzed reaction/ biological activity
(S)-malate + NAD+ --> H+ + NADH + oxaloacetate (according to UniProt)
Protein family
LDH/MDH superfamily (with Ldh, according to UniProt)
NAD+
Structure
3TL2 (PDB) (from B. anthracis, 85% identity)
Modification
phosphorylated on Arg-156 PubMed
phosphorylation on Ser-149 PubMed
Effectors of protein activity
Inhibited by Mg2+, Ca2+, Zn2+, Ag2+ and Hg2+ PubMed PubMed
Inhibited by oxaloacetate (above 1mM) and malate (above 7,7mM) PubMed
cytoplasm (according to UniProt)
membrane associated PubMed
Additional information
The enzyme is a tetramer PubMed
extensive information on the structure and enzymatic properties of Mdh can be found at Proteopedia
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
Description
Regulation
''citZ'': catabolite repression (CcpA) PubMed
Regulatory mechanism
CcpA: repression, CcpC: transcription repression PubMed, in ccpA regulon
CcpC: repression, (molecular inducer: citrate) PubMed, in ccpC regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

icdmdh

2024-12-12 10:19:40

ghost

106

f9ad3d456cafa7c9074216bd662b61b54e3995fc

2BD9E142645FECA776F7762A7431A0FB5A5D9070

Genes
Description
Regulation
''CitZ'': catabolite repression (CcpA) PubMed
Regulatory mechanism
CcpA: repression, CcpC: transcription repression PubMed, in ccpA regulon
CcpC: repression, (molecular inducer: citrate) PubMed, in ccpC regulon
CitB: mRNA stability control, upon citrate accumulation or iron limitation PubMed, in citB regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

citZmdh

2024-12-11 13:37:44

Jstuelk

118

d65e97dee2d196244ee89dd42bbf730b7ea9c870

F01A9D3586F0AD300B6C4F086E56F8AEA52D457F

Biological materials
Mutant
GP719(spc) & GP1150(spc), available in Jörg Stülke's lab PubMed
GP790 (''citZ-icd-mdh)::kan'', available in Jörg Stülke's lab
GP2331 (''citZ-icd-mdh)::kan, Cre-recombinase is integrated in sacA'',available in Jörg Stülke's lab
GP2333 (''citZ-icd-mdh)::lox72, Cre-recombinase is integrated in sacA'', available in Jörg Stülke's lab
BKE29120 (mdh::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCTCTCTTCTCCTTTA, downstream forward: _UP4_TAATAAAAAGAGAGAAAGGC
BKK29120 (mdh::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTCTCTCTTCTCCTTTA, downstream forward: _UP4_TAATAAAAAGAGAGAAAGGC
Expression vectors
pGP385: for expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
pGP1123 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab) PubMed
pGP1755 (expression / purification of Mdh-S149A, with N-terminal His-tag from E. coli, in pWH844), available in Jörg Stülke's lab
pGP1764 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab)
GP1438(mdh-Strep (spc)) & GP1440(mdh-Strep (cat)), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP1130 (spc, based on pGP1331), available in Jörg Stülke's lab PubMed
Antibody
**
GFP fusion
GP1431 (spc, based on pGP1870), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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Page visits: 8663

Time of last update: 2024-12-20 17:37:16

Author of last update: Jstuelk