mdh
168
malate dehydrogenase
Locus
BSU_29120
Molecular weight
33.49 kDa
Isoelectric point
4.73
Function
TCA cycle
Product
malate dehydrogenase
Essential
no
E.C.
1.1.1.37
Synonyms
mdh, citH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0039 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,978,734 2,979,672
The protein
Catalyzed reaction/ biological activity
(S)-malate + NAD+ --> H+ + NADH + oxaloacetate (according to UniProt)
Protein family
LDH/MDH superfamily (with Ldh, according to UniProt)
NAD+
Structure
3TL2 (PDB) (from B. anthracis, 85% identity)
Modification
Effectors of protein activity
Inhibited by oxaloacetate (above 1mM) and malate (above 7,7mM) PubMed
cytoplasm (according to UniProt)
membrane associated PubMed
Additional information
The enzyme is a tetramer PubMed
extensive information on the structure and enzymatic properties of Mdh can be found at Proteopedia
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Biological materials
Mutant
GP719(spc) & GP1150(spc), available in Jörg Stülke's lab PubMed
GP2331 (''citZ-icd-mdh)::kan, Cre-recombinase is integrated in sacA'',available in Jörg Stülke's lab
GP2333 (''citZ-icd-mdh)::lox72, Cre-recombinase is integrated in sacA'', available in Jörg Stülke's lab
Expression vectors
pGP385: for expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
pGP1123 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab) PubMed
pGP1755 (expression / purification of Mdh-S149A, with N-terminal His-tag from E. coli, in pWH844), available in Jörg Stülke's lab
pGP1764 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab)
GP1438(mdh-Strep (spc)) & GP1440(mdh-Strep (cat)), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
Antibody
**
GFP fusion
GP1431 (spc, based on pGP1870), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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Time of last update: 2024-12-20 17:37:16
Author of last update: Jstuelk