yngI

yngI
168

aceto-acetyl-CoA ligase, may be involved in surfactin production

Locus
BSU_18250
Molecular weight
61.26 kDa
Isoelectric point
5.98
Protein length
549 aaSequenceBlast
Gene length
1650 bpSequenceBlast
Function
mother cell metabolism, leucine utilization
Product
aceto-acetyl-CoA ligase
Essential
no
E.C.
6.2.1.16
Synonyms
yngI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,954,525  1,956,174
The protein
Catalyzed reaction/ biological activity
ATP + acetoacetate + CoA <=> AMP + diphosphate + acetoacetyl-CoA PubMed
Protein family
ATP-dependent AMP-binding enzyme family (according to UniProt)
Structure
3R44 (PDB) (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 32% identity) PubMed
O31826 (AlphaFold)
Paralogous protein(s)
LcfA, LcfB, YhfT
Expression and Regulation
Operons
Genes
yngJ-yngI-yngH-yngHB-yngG-yngF-yngE
Description
PubMed
Regulation
expressed during sporulation in the mother cell (SigE) PubMed
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
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yngJ→yngE

2025-04-01 23:55:18

ghost

96

ec5ff58c486f073be542df6cbb7bc2ebb4e5deee

AC16F1F9497F11969688D82F5CC45D78083A44DD

Biological materials
Mutant
MGNA-B087 (yngI::erm), available at the NBRP B. subtilis, Japan
available in Mohamed Marahiel's lab PubMed
BKE18250 (yngI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC,  downstream forward: _UP4_TCATCAAGTCAATGAGGTGA
BKK18250 (yngI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCATTGTTCCTCCTTTC,  downstream forward: _UP4_TCATCAAGTCAATGAGGTGA
References
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Page visits: 4824

Time of last update: 2025-04-08 21:59:38

Author of last update: Jstuelk