citS

citS
168

two-component sensor kinase, regulation of citrate uptake

Locus
BSU_07580
Molecular weight
59.72 kDa
Isoelectric point
6.48
Protein length
542 aaSequenceBlast
Gene length
1629 bpSequenceBlast
Function
regulation of citrate uptake
Product
two-component sensor kinase
Essential
no
Synonyms
citS, yflR
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3290 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
830,945  832,573
Phenotypes of a mutant
no growth with citrate as sole carbon source PubMed
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of CitT
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
Domains
two transmembrane segments
PAS domain
Histidine kinase domain (aa 336-528) (according to UniProt)
Structure
O34427 (AlphaFold)
Modification
autophosphorylation on a His residue
Effectors of protein activity
activity may be controlled by interaction with YflP PubMed
Localization
cell membrane (Heterogeneous) PubMed
Expression and Regulation
Operons
Genes
citS-citT-yflP
Description
PubMed
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citS→yflP

2025-04-07 21:44:02

ghost

86

48338d903fc735b45d81f8161ce84e9f939ab895

A343A4499D2510EF9EAD9C7890ADE3F0FA94FC7F

Biological materials
Mutant
MGNA-C333 (citS::erm), available at the NBRP B. subtilis, Japan
BKE07580 (citS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGCACCAGCCGTCCTTT,  downstream forward: _UP4_AAGGAGAAACAAAGGGGGAA
BKK07580 (citS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGCACCAGCCGTCCTTT,  downstream forward: _UP4_AAGGAGAAACAAAGGGGGAA
References
Reviews
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Original Publications
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7EBB09F8FF4C2FBE35B4689B15D89FEF3D58A227

Page visits: 3137

Time of last update: 2025-04-14 06:17:01

Author of last update: Melvin.boenninger