eno

eno
168

enolase, glycolytic/ gluconeogenic enzyme, universally conserved protein

Locus
BSU_33900
Molecular weight
46.42 kDa
Isoelectric point
4.49
Protein length
430 aaSequenceBlast
Gene length
1293 bpSequenceBlast
Function
enzyme in glycolysis/ gluconeogenesis
Product
enolase
Essential
Yes
E.C.
4.2.1.11
Synonyms
eno
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0148 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,476,555 3,477,847
Phenotypes of a mutant
no growth on LB, requires glucose and malate PubMed
essential according to Kobayashi et al. on LB PubMed
The protein
Catalyzed reaction/ biological activity
(2R)-2-phosphoglycerate --> H2O + PEP (according to UniProt)
Protein family
Enolase family (single member, according to UniProt)
Domains
substrate binding domain (366369)
Cofactors
Mg2+
Structure
4A3R (PDB) PubMed
1W6T (PDB) (from Streptococcus pneumoniae) PubMed
P37869 (AlphaFold)
Modification
phosphorylation on Thr-141 AND Ser-259 AND Tyr-281 AND Ser-325 PubMed
Effectors of protein activity
Inhibited by EDTA PubMed
Kinetic information
reversible Michaelis-Menten PubMed
Localization
cytoplasm PubMed
membrane associated PubMed
aggregates at the cel poles of some cells PubMed
exported, this requires a long, unbent helix (from A108 to L126) PubMed
Additional information
Enolase is a moonlighting protein PubMed
There are indications that this enzyme is an octamer PubMed
Universally conserved protein
extensive information on the structure and enzymatic properties of Eno can be found at Proteopedia
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
cggR-gapA-pgk-tpi-pgm-eno
Description
PubMed
Regulation
expression induced by glycolytic intermediates (CggR) CggR PubMed
the mRNA is processed between cggR and gapA by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Regulatory mechanism
CggR: repression, PubMed, in cggR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

cggR→eno

2025-03-27 06:06:35

Jstuelk

174

7b5a04e0f51be88bb9a430514dd0cb9d39ea3a2c

24906FF9311EDEA4AFD94747BD67B2022E642FAB

Genes
pgk-tpi-pgm-eno
Description
PubMed
Open in new tab

pgk→eno

2025-04-03 13:01:53

Jstuelk

130

c718c56bfee7b3945ef7060e9c50614ecf6b1cd7

D821B77CDDBA7B2EDE01B5B3E2E0A964498A261D

Biological materials
Mutant
GP594 (eno::cat), available in Jörg Stülke's lab, PubMed
GP698 (eno-''pgm::cat''), available in Jörg Stülke's lab, PubMed
Expression vectors
pGP1426 (expression of ''eno in B. subtilis'', in pBQ200), available in Jörg Stülke's lab
pGP1500 (expression of ''pgm and eno in B. subtilis'', in pBQ200), available in Jörg Stülke's lab
pGP563 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab, PubMed
pGP1276 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
pGP93 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab, PubMed
GP1215 (chromosomal eno-Strep fusion, spc), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed
FLAG-tag construct
GP1214 (spc, based on pGP1331), available in Jörg Stülke's lab PubMed
LacZ fusion
see pgk
GFP fusion
pHT315-yfp-eno, available in Mijakovic lab
GP1700 (in pBP43), expression of eno-GFP::spc under the native promoter, available in Jörg Stülke's lab PubMed
Labs working on this gene/protein
Jörg Stülke, University of Göttingen, Germany Homepage
References
Reviews
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Original Publications
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Subcellular localization of enolase
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Time of last update: 2025-04-03 22:24:39

Author of last update: Jstuelk