tsaD
168

required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein

Locus

BSU_05940

Molecular weight

36.69 kDa

Isoelectric point

5.02

Protein length

346 aaSequenceBlast

Gene length

1041 bpSequenceBlast

Function

tRNA modification

Product

tRNA modification enzyme

Essential

yes

E.C.

2.3.1.234

Synonyms

tsaD, ydiE, gcp

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0533 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

643,258 644,298

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed

adenosine³⁷ in tRNA + L-threonylcarbamoyladenylate --> AMP + H⁺ + N⁶-L-threonylcarbamoyladenosine³⁷ in tRNA (according to UniProt)

Protein family

KAE1 / TsaD family (with TsaB, according to UniProt)

Structure

3ZET (PDB) (from Salmonella typhimurium, 46% identity) PubMed

O05518 (AlphaFold)

Modification

in vitro phosphorylated by TsaE PubMed

Localization

cytoplasm (according to UniProt)

Expression and Regulation

Operons