lipL
168

N-amidotransferase, transfers lipoic acid from lipoyl-GcvH to AcoC, BkdB, OdhB, and PdhC, and from lipoyl-OdhB to BkdB and PdhC

Locus

BSU_37640

Molecular weight

31.27 kDa

Isoelectric point

5.27

Protein length

281 aaSequenceBlast

Gene length

846 bpSequenceBlast

Function

lipoic acid metabolism

Product

protein N-octanoyltransferase

Essential

E.C.

2.3.1.204

Synonyms

lipL, ipa-90d, ywfL

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0095 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

3,863,415 → 3,864,260

The protein

Catalyzed reaction/ biological activity

transfers lipoic acid from lipoyl-GcvH to BkdB, OdhB, and PdhC, and from lipoyl-OdhB to BkdB and PdhC PubMed

[glycine-cleavage complex H protein]-N⁶-octanoyl-L-lysine + [lipoyl-carrier protein]-L-lysine --> [glycine-cleavage complex H protein]-L-lysine + [lipoyl-carrier protein]-N⁶-octanoyl-L-lysine (according to UniProt)

Protein family

Octanoyltransferase LipL family (single member, according to UniProt)

Domains

BPL/LPL catalytic domain (aa 45-253) (according to UniProt)

Structure

2P5I (PDB) (from Bacillus halodurans, 56% identity)

P39648 (AlphaFold)

Expression and Regulation

Operons

Genes

lipL

Description

PubMed

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Biological materials

Mutant

MGNA-B667 (ywfL::erm), available at the NBRP B. subtilis, Japan

BKE37640 (ΔlipL::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATGTAAAACCCTTTC, downstream forward: _UP4_TGAACGTTCTTCGCATGCCG

BKK37640 (ΔlipL::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATGTAAAACCCTTTC, downstream forward: _UP4_TGAACGTTCTTCGCATGCCG

References

Reviews

Original Publications

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Time of last update: 2025-04-09 11:15:48

Author of last update: Jstuelk

lipL 168

lipL
168