OpuAA

• Description: glycine betaine ABC transporter (ATP-binding protein)
  
  

Gene name	opuAA
Synonyms
Essential	no
Product	glycine betaine ABC transporter (ATP-binding protein)
Function	compatible solute transport
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	46 kDa, 5.107
Gene length, protein length	1254 bp, 418 aa
Immediate neighbours	yceK, opuAB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU02980

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + quaternary amine(Out) = ADP + phosphate + quaternary amine(In) (according to Swiss-Prot)

• Protein family: ABC transporter domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P46920

• KEGG entry: [2]

• E.C. number: 3.6.3.32

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Erhard Bremer, University of Marburg, Germany homepage

References

1. Kempf B, Bremer E. (1995) OpuA, an osmotically regulated binding protein-dependent transport system for the osmoprotectant glycine betaine in Bacillus subtilis. J Biol Chem. Jul 14;270(28): 16701-13. PubMed
2. Horn C, Bremer E, Schmitt L. (2005) Functional overexpression and in vitro re-association of OpuA, an osmotically regulated ABC-transport complex from Bacillus subtilis. FEBS Lett. Oct 24;579(25): 5765-8. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed