PdxK

• Description: pyridoxal kinase
  
  

Gene name	thiD
Synonyms	ywdB, ipa-52r, pdxK
Essential	no
Product	pyridoxal kinase
Function	biosynthesis of pyridoxal phosphate
MW, pI	28 kDa, 4.922
Gene length, protein length	813 bp, 271 aa
Immediate neighbours	ywdD, ywdA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38020

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine (according to Swiss-Prot)

• Protein family: thiD family (according to Swiss-Prot)

• Paralogous protein(s): YjbV

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2IB5

• Swiss prot entry: P39610

• KEGG entry: [3]

• E.C. number: 2.7.4.7

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Joseph A Newman, Sanjan K Das, Svetlana E Sedelnikova, David W Rice
Cloning, purification and preliminary crystallographic analysis of a putative pyridoxal kinase from Bacillus subtilis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2006, 62(Pt 10);1006-9
[PubMed:17012797] [WorldCat.org] [DOI] (I p)

Joo-Heon Park, Kristin Burns, Cynthia Kinsland, Tadhg P Begley
Characterization of two kinases involved in thiamine pyrophosphate and pyridoxal phosphate biosynthesis in Bacillus subtilis: 4-amino-5-hydroxymethyl-2methylpyrimidine kinase and pyridoxal kinase.
J Bacteriol: 2004, 186(5);1571-3
[PubMed:14973012] [WorldCat.org] [DOI] (P p)

1. Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed