Difference between revisions of "Obg"
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− | <pubmed>12427945, </pubmed> | + | <pubmed>15325267,18689482,7961487,7961486,15583165,2537815,10781545,10419966,12427945, </pubmed> |
# Morimoto T, Loh PC, Hirai T, Asai K, Kobayashi K, Moriya S, Ogasawara N (2002) Six GTP-binding proteins of the Era/Obg family are essential for cell growth in ''Bacillus subtilis''. ''Microbiology'' '''148:''' 3539-3552. [http://www.ncbi.nlm.nih.gov/sites/entrez/12427945 PubMed] | # Morimoto T, Loh PC, Hirai T, Asai K, Kobayashi K, Moriya S, Ogasawara N (2002) Six GTP-binding proteins of the Era/Obg family are essential for cell growth in ''Bacillus subtilis''. ''Microbiology'' '''148:''' 3539-3552. [http://www.ncbi.nlm.nih.gov/sites/entrez/12427945 PubMed] | ||
# Buglino J., Shen V., Hakiman P. & Lima C.D. (2002) Structural and biochemical analysis of the Obg GTP binding protein ''Structure'' '''10:''' 1581-1592. [http://www.ncbi.nlm.nih.gov/pubmed/12429099?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed] | # Buglino J., Shen V., Hakiman P. & Lima C.D. (2002) Structural and biochemical analysis of the Obg GTP binding protein ''Structure'' '''10:''' 1581-1592. [http://www.ncbi.nlm.nih.gov/pubmed/12429099?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed] | ||
# Scott, J.M., Ju, J., Mitchell, T., and Haldenwang, W.G. (2000) The Bacillus subtilis GTP binding protein obg and regulators of the sigma(B) stress response transcription factor cofractionate with ribosomes ''J. Bacteriol.'' '''182:''' 2771–2777. [http://www.ncbi.nlm.nih.gov/pubmed/10781545?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed] | # Scott, J.M., Ju, J., Mitchell, T., and Haldenwang, W.G. (2000) The Bacillus subtilis GTP binding protein obg and regulators of the sigma(B) stress response transcription factor cofractionate with ribosomes ''J. Bacteriol.'' '''182:''' 2771–2777. [http://www.ncbi.nlm.nih.gov/pubmed/10781545?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum PubMed] |
Revision as of 17:15, 13 June 2009
- Description: GTP-binding protein involved in initiation of sporulation
Gene name | obg |
Synonyms | |
Essential | Yes |
Product | GTP-binding protein |
Function | may be required to stimulate activity of the phosphorelay that activates Spo0A |
MW, pI | 47 kDa, 4.859 |
Gene length, protein length | 1284 bp, 428 aa |
Immediate neighbours | spo0B, pheB |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU27920
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
- Protein family: GTP1/OBG family (according to Swiss-Prot) Era/Obg family
Extended information on the protein
- Kinetic information:
- Domains:
Obg possesses 3 domains: A N-terminal glycine-rich domain (1–158), a GTP binding domain (159–342) and a C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
- Modification:
- Cofactor(s):
GDP, GTP and probably ppGpp
- Effectors of protein activity:
- Interactions:
Interaction with Rsb proteins.
- Localization:
Database entries
- Swiss prot entry: P20964
- KEGG entry: [3]
- E.C. number:
Additional information
The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.
Expression and regulation
- Sigma factor: SigA
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector: pGP835 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Bill Haldenwang, San Antonio, USA
- Naotake Ogasawara, Nara, Japan
Your additional remarks
References
- Morimoto T, Loh PC, Hirai T, Asai K, Kobayashi K, Moriya S, Ogasawara N (2002) Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis. Microbiology 148: 3539-3552. PubMed
- Buglino J., Shen V., Hakiman P. & Lima C.D. (2002) Structural and biochemical analysis of the Obg GTP binding protein Structure 10: 1581-1592. PubMed
- Scott, J.M., Ju, J., Mitchell, T., and Haldenwang, W.G. (2000) The Bacillus subtilis GTP binding protein obg and regulators of the sigma(B) stress response transcription factor cofractionate with ribosomes J. Bacteriol. 182: 2771–2777. PubMed