SepF

• Description: part of the divisome
  
  

Gene name	sepF
Synonyms	ylmF
Essential	no
Product	FtsZ-interacting protein
Function	proper execution of septum synthesis
MW, pI	17 kDa, 4.863
Gene length, protein length	447 bp, 149 aa
Immediate neighbours	ylmE, ylmG
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU15390

Phenotypes of a mutant

Less efficient cell division results in longer cells. Electron microscopy reveals strongly distorted division septa.

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

Cell division

• Protein family: sepF family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SepF-FtsZ

• Localization: cytoplasm (according to Swiss-Prot), Septum

Database entries

• Structure:

• Swiss prot entry: O31728

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Leendert Hamoen, CBCB, Newcastle University, UK

Your additional remarks

SepF mutation is synthetic lethal in combination with an ezrA mutation or an ftsA mutation.

References

1. Hamoen et al. (2006) SepF, a novel FtsZ-interacting protein required for a late step in cell division Mol. Microbiol. 59(3): 989-99. Pubmed
2. Ishikawa et al. (2006) A new FtsZ-interacting protein, YlmF, complements the activity of FtsA during progression of cell division in Bacillus subtilis Mol. Microbiol. 60(6): 1364-80. Pubmed