DltA

• Description: D-alanyl-D-alanine carrier protein ligase
  
  

Gene name	dltA
Synonyms	ipa-5r, dae
Essential	no
Product	D-alanyl-D-alanine carrier protein ligase
Function	biosynthesis of teichoic acid
MW, pI	55 kDa, 4.929
Gene length, protein length	1509 bp, 503 aa
Immediate neighbours	menA, dltB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU38500

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate) (according to Swiss-Prot)

• Protein family: DltA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3E7X

• Swiss prot entry: P39581

• KEGG entry: [3]

• E.C. number: 6.1.1.13

Expression and regulation

• Operon: dltA-dltB-dltC-dltD-dltE

• Sigma factor: SigD, SigX

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism: activated by YvrHb PubMed
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

References

Khan Tanjid Osman, Liqin Du, Yujiong He, Yu Luo
Crystal structure of Bacillus cereus D-alanyl carrier protein ligase (DltA) in complex with ATP.
J Mol Biol: 2009, 388(2);345-55
[PubMed:19324056] [WorldCat.org] [DOI] (I p)

Masakuni Serizawa, Keisuke Kodama, Hiroki Yamamoto, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Functional analysis of the YvrGHb two-component system of Bacillus subtilis: identification of the regulated genes by DNA microarray and northern blot analyses.
Biosci Biotechnol Biochem: 2005, 69(11);2155-69
[PubMed:16306698] [WorldCat.org] [DOI] (P p)

M Perego, P Glaser, A Minutello, M A Strauch, K Leopold, W Fischer
Incorporation of D-alanine into lipoteichoic acid and wall teichoic acid in Bacillus subtilis. Identification of genes and regulation.
J Biol Chem: 1995, 270(26);15598-606
[PubMed:7797557] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed