NrdE

• Description: ribonucleoside-diphosphate reductase (major subunit)
  
  

Gene name	nrdE
Synonyms
Essential	yes PubMed
Product	ribonucleoside-diphosphate reductase (major subunit)
Function	synthesis of deoxyribonucleoside triphosphates
MW, pI	80 kDa, 5.931
Gene length, protein length	2100 bp, 700 aa
Immediate neighbours	nrdI, nrdF
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU17380

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H₂O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)

• Protein family: ribonucleoside diphosphate reductase large chain family (according to Swiss-Prot)

• Paralogous protein(s): YosN

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NrdE-NrdF PubMed

• Localization:

Database entries

• Structure:

• Swiss prot entry: P50620

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: nrdI-nrdE-nrdF-ymaB PubMed

• Sigma factor:

• Regulation: distinct promoters for aerobic and anaerobic expression PubMed, anaerobic expression requires ResD PubMed
  • expressed under anaerobic conditions (ResD) PubMed

• Regulatory mechanism: ResD: transcription activation PubMed
  • ResD: transcription activation PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Elisabeth Härtig, Braunschweig, Germany Homepage

References

Elisabeth Härtig, Anja Hartmann, Manuela Schätzle, Alessandra M Albertini, Dieter Jahn
The Bacillus subtilis nrdEF genes, encoding a class Ib ribonucleotide reductase, are essential for aerobic and anaerobic growth.
Appl Environ Microbiol: 2006, 72(8);5260-5
[PubMed:16885274] [WorldCat.org] [DOI] (P p)

1. Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed