HrcA

• Description: transcriptional repressor of class I heat-shock genes
  
  

Gene name	hrcA
Synonyms	yqxE, yqeS
Essential	no
Product	transcriptional repressor
Function	regulation of chaperone gene expression
MW, pI	38 kDa, 6.079
Gene length, protein length	1029 bp, 343 aa
Immediate neighbours	grpE, hemN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU25490

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repressor of the hrcA-grpE-dnaK-dnaJ and groES-groEL operons (at low/ normal temperatures)

• Protein family: hrcA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): GroEL acts as co-repressor

• Effectors of protein activity:

• Interactions: GroEL-HrcA

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P25499

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by heat shock (HrcA) PubMed

• Regulatory mechanism: repressed at normal temperature by HrcA

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage

Thomas Wiegert, University of Bayreuth, Germany Homepage

References

A Mogk, G Homuth, C Scholz, L Kim, F X Schmid, W Schumann
The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis.
EMBO J: 1997, 16(15);4579-90
[PubMed:9303302] [WorldCat.org] [DOI] (P p)

M Wetzstein, U Völker, J Dedio, S Löbau, U Zuber, M Schiesswohl, C Herget, M Hecker, W Schumann
Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis.
J Bacteriol: 1992, 174(10);3300-10
[PubMed:1339421] [WorldCat.org] [DOI] (P p)

1. Wetzstein, M., U. Völker, J. Dedio, S. Löbau, U. Zuber, M. Schiesswohl, C. Herget, M. Hecker, and W. Schumann. 1992. Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis. J. Bacteriol. 174:3300-3310. PubMed
2. Mogk, A., Homuth, G., Scholz, C., Kim, L., Schmid, F. X., and Schumann, W. (1997) The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis. EMBO J 16, 4579-4590. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed