Difference between revisions of "PdhB"
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=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU14590 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 81: | Line 81: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P21882 P21882] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P21882 P21882] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU14590] |
* '''E.C. number:''' [http://www.expasy.org/enzyme/1.2.4.1 1.2.4.1] | * '''E.C. number:''' [http://www.expasy.org/enzyme/1.2.4.1 1.2.4.1] | ||
Revision as of 14:04, 3 June 2009
- Description: pyruvate dehydrogenase (E1 beta subunit)
| Gene name | pdhB |
| Synonyms | |
| Essential | no |
| Product | pyruvate dehydrogenase (E1 beta subunit) |
| Function | links glycolysis and TCA cycle |
| MW, pI | 35 kDa, 4.547 |
| Gene length, protein length | 975 bp, 325 aa |
| Immediate neighbours | pdhA, pdhC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU14590
Phenotypes of a mutant
defects in sporulation and unable to grow on glucose as single carbon source PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 (according to Swiss-Prot)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylation on (Ser-302 OR Ser-306) PubMed
- Cofactor(s):
- Effectors of protein activity:
- Localization: membrane associated PubMed
Database entries
- Structure: 1W88 (E1 in complex with subunit binding domain of E2, Geobacillus stearothermophilus)
- Swiss prot entry: P21882
- KEGG entry: [3]
- E.C. number: 1.2.4.1
Additional information
Expression and regulation
- Sigma factor: SigA
- Regulation: expression activated by glucose (2.8 fold) PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP459 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Arthur Aronson, Purdue University, West Lafayette, USA homepage
Your additional remarks
References
- Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
- Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
- Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
- Gao et al. (2002) The E1beta and E2 subunits of the Bacillus subtilis pyruvate dehydrogenase complex are involved in regulation of sporulation.J. Bacteriol. 184: 2780-2788. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
