Difference between revisions of "Rny"
| Line 37: | Line 37: | ||
=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU16960 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 86: | Line 86: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O31774 O31774] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O31774 O31774] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU16960] |
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.4.16 3.1.4.16] 6 3.1.4.16] | * '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.4.16 3.1.4.16] 6 3.1.4.16] | ||
Revision as of 09:59, 3 June 2009
- Description: RNase Y, endoribonuclease, required for the processing of the gapA operon mRNA
| Gene name | rny |
| Synonyms | ymdA |
| Essential | yes |
| Product | RNase Y |
| Function | required for the processing of the gapA operon mRNA |
| MW, pI | 58,7 kDa, 5.39 |
| Gene length, protein length | 1560 bp, 520 amino acids |
| Immediate neighbours | pbpX, ymdB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU16960
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 3'-phosphate (according to Swiss-Prot) required for the processing of the gapA operon mRNA PubMed
- Protein family: 2',3' cyclic nucleotide phosphodiesterase family (according to Swiss-Prot) 2',3' cyclic nucleotide phosphodiesterase family
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- transmembrane domain (4–24)
- KH domain (210–273)
- HD domain (336–429)
- Modification:
- Cofactor(s): metal-dependent
- Effectors of protein activity:
Database entries
- Structure:
- Swiss prot entry: O31774
- KEGG entry: [2]
- E.C. number: 3.1.4.16 6 3.1.4.16]
Additional information
required for the processing of the gapA operon mRNA
Expression and regulation
- Sigma factor:
- Regulation: constitutive
- Regulatory mechanism:
- Additional information: there is a terminator between rny and ymdB, most transcripts terminate there
Biological materials
- Mutant: essential!!!!, 4043 (rny under p-spac control), GP193 (rny under p-xyl control), available in Stülke lab
- Expression vector:
N-terminal Strep-tag, expression in E. coli, in pGP172: pGP441, available in Stülke lab
N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380: pGP775 , available in Stülke lab
wild type rny, expression in B. subtilis, in pBQ200: pGP1201, available in Stülke lab
there is also a series of domain constructs present in pBQ200, all available in Stülke lab
- GFP fusion: B. subtilis 3569 (amyE:: (p-xyl rny-gfpmut1-spc)), available in Errington lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
Labs working on this gene/protein
Harald Putzer, IBPC Paris, France Homepage
Jörg Stülke, University of Göttingen, Germany Homepage
Your additional remarks
References
