TufA

• Description: elongation factor Tu
  
  

Gene name	tufA
Synonyms
Essential	yes PubMed
Product	elongation factor Tu
Function	translation
MW, pI	43 kDa, 4.723
Gene length, protein length	1188 bp, 396 aa
Immediate neighbours	fusA, ybaC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: EF-Tu/EF-1A subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on Thr-384 by PrkC, dephosphorylated by PrpC PubMed, PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot), membrane PubMed

Database entries

• Structure:

• Swiss prot entry: P33166

• KEGG entry: BSU01130

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector: pGP839 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed