Difference between revisions of "HrcA"
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* '''Modification:''' | * '''Modification:''' | ||
| − | * '''Cofactor(s):''' | + | * '''Cofactor(s):''' [[GroEL]] acts as co-repressor |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
Revision as of 07:17, 31 May 2009
- Description: transcriptional repressor of class I heat-shock genes
| Gene name | hrcA |
| Synonyms | yqxE, yqeS |
| Essential | no |
| Product | transcriptional repressor |
| Function | regulation of chaperone gene expression |
| MW, pI | 38 kDa, 6.079 |
| Gene length, protein length | 1029 bp, 343 aa |
| Immediate neighbours | grpE, hemN |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcription repressor of the hrcA-grpE-dnaK-dnaJ and groES-groEL operons (at low/ normal temperatures)
- Protein family: hrcA family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): GroEL acts as co-repressor
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: P25499
- KEGG entry: BSU25490
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism: repressed at normal temperature by HrcA
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Wolfgang Schumann, Bayreuth University, Germany Homepage
Thomas Wiegert, University of Bayreuth, Germany Homepage
Your additional remarks
References
- Wetzstein, M., U. Völker, J. Dedio, S. Löbau, U. Zuber, M. Schiesswohl, C. Herget, M. Hecker, and W. Schumann. 1992. Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis. J. Bacteriol. 174:3300-3310. PubMed
- Mogk, A., Homuth, G., Scholz, C., Kim, L., Schmid, F. X., and Schumann, W. (1997) The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis. EMBO J 16, 4579-4590. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
