Difference between revisions of "CapB"

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* '''Description:''' similar to capsular polyglutamate biosynthesis <br/><br/>
+
* '''Description:''' capsular polyglutamate biosynthesis <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|''capB''
 
|''capB''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywsC''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywsC, pgsB''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
+
|style="background:#ABCDEF;" align="center"| '''Product''' || poly-gamma-glutamate synthetase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
+
|style="background:#ABCDEF;" align="center"|'''Function''' || capsule synthesis
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 5.073   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 5.073   
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
 +
no synthesis of the poly-gamma-glutamate capsule [http://www.ncbi.nlm.nih.gov/sites/entrez/11751809 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' synthesis of poly-gamma-glutamate from L-Glu [http://www.ncbi.nlm.nih.gov/sites/entrez/11751809 PubMed]
  
 
* '''Protein family:'''
 
* '''Protein family:'''
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[capB]]-[[capC]]-[[capA]]''
  
 
* '''Sigma factor:'''  
 
* '''Sigma factor:'''  
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* '''Regulation:'''  
 
* '''Regulation:'''  
  
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' activated by [[DegU]]-P
  
* '''Additional information:'''  
+
* '''Additional information:''' The operon is not expressed in the lab strain 168 due to lack of expression of [[DegQ]] resulting in inactivity of [[DegS]] and [[DegU]]
  
 
=Biological materials =
 
=Biological materials =
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* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  
  
* '''Antibody:'''
+
* '''Antibody:'''  
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 09:27, 21 May 2009

  • Description: capsular polyglutamate biosynthesis

Gene name capB
Synonyms ywsC, pgsB
Essential no
Product poly-gamma-glutamate synthetase
Function capsule synthesis
MW, pI 43 kDa, 5.073
Gene length, protein length 1179 bp, 393 aa
Immediate neighbours capC, rbsR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwsC context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

no synthesis of the poly-gamma-glutamate capsule PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: synthesis of poly-gamma-glutamate from L-Glu PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism: activated by DegU-P
  • Additional information: The operon is not expressed in the lab strain 168 due to lack of expression of DegQ resulting in inactivity of DegS and DegU

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed