Difference between revisions of "PGP570"
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| + | Vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein, after purification the tag can be cleaved off by thrombin | ||
| − | + | the [[plasmid]] carries the ''lacI'' gene, regulation is possible in any ''E. coli'' strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | |
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| − | the plasmid carries the ''lacI'' gene, regulation is possible in any ''E. coli'' strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | ||
constructed in the [[Stülke]] lab | constructed in the [[Stülke]] lab | ||
Revision as of 12:00, 16 April 2009
Vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein, after purification the tag can be cleaved off by thrombin
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
based on pWH844
in E. coli: ampicillin resistance
unpublished
