Difference between revisions of "PGP570"
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| − | + | Vector for the expression of proteins in ''E. coli'', the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein, after purification the tag can be cleaved off by thrombin | |
the plasmid carries the ''lacI'' gene, regulation is possible in any ''E. coli'' strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | the plasmid carries the ''lacI'' gene, regulation is possible in any ''E. coli'' strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | ||
Revision as of 11:33, 16 April 2009
Vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein, after purification the tag can be cleaved off by thrombin
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Stülke lab
based on pWH844
in E. coli: ampicillin resistance
unpublished
