LevR

• Description: transcriptional activator of the levD-levE-levF-levG-sacC operon
  
  

Gene name	levR
Synonyms
Essential	no
Product	transcriptional activator, PRD-type (for SigL-dependent promoter)
Function	regulation of levan and fructose utilization
MW, pI	105 kDa, 5.943
Gene length, protein length	2814 bp, 938 aa
Immediate neighbours	levD, aapA
Gene sequence (+200bp)	Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binding to the upstream activating sequence in front of the levD gene results in activation of transcription of the levD-levE-levF-levG-sacC operon, bining occurs in the presence of fructose

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Débarbouillé M, Martin-Verstraete I, Klier A, Rapoport G (1991) The transcriptional regulator LevR of Bacillus subtilis has domains homologous to both (Sigma)54 - and phosphotransferase system-dependent regulators. Proc Natl Acad Sci USA 88:2212-2216. PubMed
2. Stülke, J., Martin-Verstraete, I., Charrier, V., Klier, A., Deutscher, J. & Rapoport, G. (1995) The HPr protein of the phosphotransferase system links induction and catabolite repression of the Bacillus subtilis levanase operon. J. Bacteriol. 177: 6928-6936. PubMed
3. Martin-Verstraete, I., Stülke, J., Klier, A. & Rapoport, G. (1995) Two different mechanisms mediate catabolite repression of the Bacillus subtilis levanase operon. J. Bacteriol. 177: 6919-6927. PubMed
4. Martin-Verstraete, I., Charrier, V., Stülke, J., Galinier, A., Erni, B., Rapoport, G., & Deutscher, J. (1998) Antagonistic effects of dual PTS catalyzed phosphorylation on the Bacillus subtilis transcriptional activator LevR. Mol. Microbiol. 28: 293-303. PubMed
5. Martin-Verstraete I, Débarbouillé M, Klier A, Rapoport G (1994) Interactions of wild type and truncated LevR of Bacillus subtilis with the upstream activating sequence of the levanase operon. J Mol Biol 241:178-192. PubMed
6. Charrier V, Deutscher J, Martin-Verstraete I (1997b) Protein phosphorylation chain of a Bacillus subtilis fructose-specific phosphotransferase system and its participation in regulation of the expression of the lev operon. Biochemistry 36:1163-1172. PubMed
7. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed