Difference between revisions of "PGP380"

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[[File:PGP380.jpeg|250px|thumb|right|'''pGP380: click to enlarge''']]
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Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the SPINE technology
 
Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the SPINE technology
  

Revision as of 15:23, 17 March 2009

pGP380: click to enlarge

Vector for the overexpression of N-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology

constructed in Stülke lab

in E. coli: ampicillin resistance

in B. subtilis: erythromycin resistance

based on pBQ200

similar vector: pGP382

Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. PubMed