Difference between revisions of "PHT10"
(Created page with "{{ContentDisclamer}} 600px 230px The pHT10 vector contains the coding sequence for a c-Myc tag and allows high-level intracellular pro...") |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 2: | Line 2: | ||
[[File:PHT10.PNG|600px]] | [[File:PHT10.PNG|600px]] | ||
| − | [[File: | + | [[File:PHT10-Legende.jpg|300px]] |
The pHT10 vector contains the coding sequence for a c-Myc tag and allows high-level intracellular production of recombinant c-Myc tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest. | The pHT10 vector contains the coding sequence for a c-Myc tag and allows high-level intracellular production of recombinant c-Myc tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest. | ||
| Line 14: | Line 14: | ||
* ''E. coli / B. subtilis'' shuttle vector | * ''E. coli / B. subtilis'' shuttle vector | ||
| − | pHT10 is a variant of [[PHT01|pHT01]], both vectors are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)] | + | pHT10 is a variant of [[PHT01|pHT01]], both vectors are available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]] |
== References == | == References == | ||
<pubmed>17624574, 16005967, 16125412</pubmed> | <pubmed>17624574, 16005967, 16125412</pubmed> | ||
Latest revision as of 11:35, 28 August 2017
Disclaimer
The content of this page/section is provided by a third party. SubtiWiki acknowledges that this third party content is obtained from a source believed to be reliable. Please note that no guarantees are made by SubtiWiki as to reliability of this third party information. Provision of this third party content is for general information purpose and does not constitute a recommendation. SubtiWiki does not own copyright over this third party content (including but not limited to text, images, and media).
The pHT10 vector contains the coding sequence for a c-Myc tag and allows high-level intracellular production of recombinant c-Myc tagged protein with B. subtilis. The tag can be used for detection or purification of the produced protein of interest. The expression is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT10 is an E. coli / B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
Features
- High level production of recombinant proteins
- Efficiently controllable gene expression (IPTG inducible)
- C-terminal c-Myc tag
- E. coli / B. subtilis shuttle vector
pHT10 is a variant of pHT01, both vectors are available from MoBiTec GmbH (Göttingen)
References
Hoang Duc Nguyen, Trang Thi Phuong Phan, Wolfgang Schumann
Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis.
Curr Microbiol: 2007, 55(2);89-93
[PubMed:17624574]
[WorldCat.org]
[DOI]
(P p)
Trang Thi Phuong Phan, Hoang Duc Nguyen, Wolfgang Schumann
Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis.
Protein Expr Purif: 2006, 46(2);189-95
[PubMed:16125412]
[WorldCat.org]
[DOI]
(P p)
Hoang Duc Nguyen, Quynh Anh Nguyen, Rita C Ferreira, Luis C S Ferreira, Linh Thuoc Tran, Wolfgang Schumann
Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability.
Plasmid: 2005, 54(3);241-8
[PubMed:16005967]
[WorldCat.org]
[DOI]
(P p)
