mgtE

mgtE
168

primary magnesium transporter

Locus
BSU_13300
Molecular weight
50.68 kDa
Isoelectric point
4.61
Protein length
451 aaSequenceBlast
Gene length
1356 bpSequenceBlast
Function
magnesium uptake
Product
primary magnesium transporter
Essential
yes
Synonyms
mgtE, ykoK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2239 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,396,013  1,397,368
Phenotypes of a mutant
essential PubMed
a mgtE mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of CitM) PubMed
a mgtE yfjQ mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of CitM) PubMed
The protein
Protein family
SLC41A transporter family (single member, according to UniProt)
Domains
2 CBS domains (aa 140-203, aa 204-260) (according to UniProt)
Structure
2YVX (PDB) (from Thermus thermophilus, 34% identity) PubMed
2QBZ (PDB) (the mgtE riboswitch)
O34442 (AlphaFold)
Effectors of protein activity
activity is inhibited by high intracellular Mg2+ concentration PubMed
binds c-di-AMP PubMed
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
mgtE
Description
PubMed
Regulation
induced at low magnesium concentration (M-box) PubMed
antitermination is modulated by NusG-dependent transcription pausing just upstream of the M-box PubMed
Regulatory mechanism
M-box: RNA switch, Mg-responsive M-box, in M-box
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mgtE

2025-02-22 08:18:22

Jstuelk

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E4E563F4357DEC2C5B5815924274C7E7C86271E2

9D504E9849A9C48B5EBBF49732CD003FA8586A9A

Additional information
MgtE stability is tightly regulated by the intramembrane protease FtsH (via the adaptor protein YqgP) PubMed
expression is increased as a result of loop duplication in L22 PubMed
Biological materials
Mutant
MGNA-B313 (mgtE::erm), available at the NBRP B. subtilis, Japan
GP2854 (ΔmgtE::ermC), available in Jörg Stülke's lab
GP2762 (ΔmgtE::spec), available in Jörg Stülke's lab
GP2763 (ΔcitM::kan ΔmgtE::spec), available in Jörg Stülke's lab
Expression vectors
pGP2927 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
FLAG-tag construct
GP2768 mgtE-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab
LacZ fusion
GP2869 (based on pAC5), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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472E3A2407C83EEE26DB00079D185C4EA2988611

Page visits: 4660

Time of last update: 2025-04-09 22:09:18

Author of last update: Jstuelk