cpgA

cpgA
168

ribosome assembly GTPase, activity stimulated by ribosomes, may be involved in the maturation of the 30S subunit of the ribosome, detoxification of 4-phosphoerythronate

Locus
BSU_15780
Molecular weight
33.64 kDa
Isoelectric point
4.74
Protein length
298 aaSequenceBlast
Gene length
897 bpSequenceBlast
Function
ribosome assembly, coordination of peptidoglycan deposition in the cell wall, metabolite proofreading
Product
GTPase
Essential
no
Synonyms
cpgA, yloQ, rsgA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1162 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,653,103  1,653,999
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
forms curly cells PubMed
poor growth PubMed
non-transformable PubMed
defective in 16S rRNA 3' processing by YqfG PubMed
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C PubMed
The protein
Catalyzed reaction/ biological activity
dephosphorylation of 4-phosphoerythronate thus preventing inhibition of GndA PubMed
Protein family
TRAFAC class YlqF/YawG GTPase family (together with RbgA and YqeH, according to UniProt)
Domains
CP-type G domain (aa 67-228) (according to UniProt)
Structure
1T9H (PDB) PubMed
O34530 (AlphaFold)
Modification
in vitro phosphorylated on Thr-166 by PrkC, dephosphorylated by PrpC PubMed
Expression and Regulation
Operons
Genes
prpC-prkC-cpgA-rpeA-yloS
Description
PubMed
Open in new tab

prpC→yloS

2025-04-04 08:05:59

Jstuelk

129

f45e3810755c3fba238243b21c1c17e072c8fe47

14D2827FA6BD60210DFADC1BB1FFF0C98DC768FA

Genes
cpgA
Description
PubMed
Expression
expression is decreased upon depletion of depletion of tRNA maturation factors rnpB or rnz PubMed
Open in new tab

cpgA

2025-03-17 08:41:06

Jstuelk

97

9B5376A22F42C49A349590080432E7A657E40BDC

A15AF0C53EF5258846124085281D05395B8422FD

Biological materials
Mutant
MGNA-B135 (yloQ::erm), available at the NBRP B. subtilis, Japan
BKE15780 (cpgA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTCAGGCATATTTTCCCTCC,  downstream forward: _UP4_GACAGAAAGCCGAGGTATTA
BKK15780 (cpgA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTCAGGCATATTTTCCCTCC,  downstream forward: _UP4_GACAGAAAGCCGAGGTATTA
BP1272 (cpgA::cat trp+) available in Jörg Stülke's & Fabian Commichau's lab, strain cannot grow with glucose, see Sachla et al (2019)
Labs working on this gene/protein
Tony Wilkinson, York University, U.K. homepage
References
Reviews
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Original Publications
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519D4694B2895EE6F0D38D918F52F27734506848

Page visits: 5561

Time of last update: 2025-04-06 05:19:34

Author of last update: Christoph.elfmann