mccA

mccA
168

O-acetylserine-thiol-lyase

Locus
BSU_27260
Molecular weight
32.86 kDa
Isoelectric point
5.23
Protein length
307 aaSequenceBlast
Gene length
921 bpSequenceBlast
Function
methionine-to-cysteine conversion
Product
O-acetylserine-thiol-lyase
Essential
no
Synonyms
mccA, yrhA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0031 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,786,142 → 2,787,065
Phenotypes of a mutant
a mccA cysK double mutant is auxotrophic for cysteine PubMed
The protein
Catalyzed reaction/ biological activity
L-homocysteine + O-acetyl-L-serine --> acetate + H+ + L,L-cystathionine (according to UniProt)
Protein family
Cysteine synthase/cystathionine beta-synthase family (with CysK and YtkP, according to UniProt)
Cofactors
PLP (according to UniProt)
Structure
4QL4 (PDB) (from B. anthracis, 63% identity)
O05393 (AlphaFold)
Paralogous protein(s)
CysK, YtkP
Localization
cytoplasm (according to UniProt)
Expression and Regulation
Operons
Genes
yrrT-mtnN-mccA-mccB-yrhC
Description
PubMed
Regulation
repressed in the presence of cysteine (CymR) PubMed
Regulatory mechanism
CymR: repression, PubMed, in cymR regulon
Spx: activation, PubMed, in spx regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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yrrT→yrhC

2025-03-16 01:20:24

ghost

125

520f018506caa674deba5a8750946c81db19309b

6F8866D3B9D23886BC36B8964568BAC6D245EA17

Biological materials
Mutant
MGNA-A849 (yrhA::erm), available at the NBRP B. subtilis, Japan
1A945 ( mccA::kan), PubMed, available at BGSC
BKE27260 (ΔmccA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATGTCCTCCTCCTTA,  downstream forward: _UP4_CAAATATACGAAGGAGGCAT
BKK27260 (ΔmccA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATATGTCCTCCTCCTTA,  downstream forward: _UP4_CAAATATACGAAGGAGGCAT
References
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Time of last update: 2025-04-05 20:01:35

Author of last update: Melvin.boenninger