dnaA
168

AAA ATPase, replication initiation protein

Locus

BSU_00010

Molecular weight

50.70 kDa

Isoelectric point

6.03

Protein length

446 aaSequenceBlast

Gene length

1338 bpSequenceBlast

Function

DNA replication

Product

replication initiation protein

Essential

yes

Synonyms

dnaA

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0593 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

410 → 1,750

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

binds multiple regions in the oriC region, required for recruitment of proteins needed to load the replicative helicase DnaC

binds to a pair of double-stranded DNA binding sites (DnaA‐boxes) that guide the protein onto adjacent ssDNA binding motifs (DnaA‐trios) where the protein assembles into an oligomer that stretches DNA to promote origin unwinding PubMed

Protein family

DnaA family (single member, according to UniProt)

Domains

Domain I: aa 1 ... 80: oligomerization and interaction with DnaB PubMed

Domain II: aa 81 ... 114: flexible loop PubMed

Domain III: aa 115 ... 353: AAA+ motif, binding of ATP, ATPase activity, second site for interaction with DnaB, local DNA unwinding PubMed

Domain IV: HTH motif for specific dsDNA binding (DnaA boxes) PubMed

Structure

8BTG (PDB) PubMed

P05648 (AlphaFold)

Effectors of protein activity

SirA displaces DnaA from the replication origin PubMed

YabA inhibits co-operative binding of DnaA to the oriC DNA PubMed

DnaA helix formation (and thus replication initiation) is inhibited by the interaction of either ParA, YabA or DnaN with the AAA domain of DnaA PubMed

interaction with DnaD inhibits the ability of DnaA to cooperatively bind to DNA PubMed

DNA gyrase (GyrA-GyrB) inhibits association of DnaA with oriC PubMed

Localization

throughout the cytoplasm PubMed

co-localizes with the origin region and replication forks throughout the cell cycle PubMed

Expression and Regulation

Operons

Genes

dnaA-dnaN

Description

PubMed

Regulation