rpoB

rpoB
168

RNA polymerase beta subunit

Locus
BSU_01070
Molecular weight
133.38 kDa
Isoelectric point
4.73
Protein length
1193 aaSequenceBlast
Gene length
3582 bpSequenceBlast
Function
transcription
Product
RNA polymerase beta subunit
Essential
yes
E.C.
2.7.7.6
Synonyms
rpoB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0085 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
121,919 → 125,500
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
Nucleoside triphosphate + RNA(n) --> diphosphate + RNA(n + 1) (according to UniProt)
Protein family
RNA polymerase beta chain family (single member, according to UniProt)
Structure
6WVJ (PDB) (RNA polymerase elongation complex, RpoA-RpoB-RpoC-YloH) PubMed
3IYD (PDB) (RNA polymerase from E. coli, 59% identity) PubMed
3MLQ (PDB) (RNA polymerase interacting domain of Thermus thermophilus Mfd with the Thermus aquaticus RpoB beta1 domain) PubMed
8EHQ (PDB) (the paused transcription complex of Mycobacterium tuberculosis RNA polymerase with B. subtilis NusG) PubMed
8EOE (PDB) (the elongation complex of Mycobacterium tuberculosis RNA polymerase with B. subtilis NusG) PubMed
P37870 (AlphaFold)
Modification
phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 PubMed
Localization
cytoplasm
Expression and Regulation
Operons
Genes
rpoB-rpoC
Description
PubMed
Regulation
Rel dependent downregulation (Class I) during stringent response PubMed
the leader mRNA is processed upstream of rpoB by RNase Y, this requires the RicA-RicF-RicT complex PubMed
Regulatory mechanism
stringent response: negative regulation, in stringent response
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

rpoB→rpoC

2025-04-04 06:36:52

Jstuelk

164

9ecfb91baa38d439fdb11db43c6bb767fe4b71dc

AA678F0994EDBB47CABD9A659CCE017143F2ECAA

Biological materials
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
FLAG-tag construct
GP2043 rpoB-3xFLAG ermC (based on pGP1087), available in Jörg Stülke's lab
LacZ fusion
pGP2683 (cat) based on pAC5, available in Jörg Stülke's lab
pGP2682 (aphA3) based on pAC7, available in Jörg Stülke's lab
References
Reviews
Loading
Original Publications
Loading

EC32328548BFB59D9026ACE351C946DBBC0932BC

Page visits: 13682

Time of last update: 2025-04-04 06:56:13

Author of last update: Jstuelk